| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
Lung-targeted gene transfer is expected to revolutionize the treatment of respiratory diseases. In this study, we demonstrated that naked plasmid deoxyribonucleic acid (DNA) can be transferred into alveolar cells in normal rats, by retrograde injection into the pulmonary vein with the left pulmonary artery clamped. When a lusiferase expression plasmid vector pCAGGS-lusiferase in a reporter assay was examined, the maximal lusiferase expression was obtained when the DNA solution was injected within 30 s in a volume of 1.0 mL. We detected lacZ expression exclusively in the type II pnumocyte attached to alveolar walls by immunoelectron microscopy. Protocol for naked plasmid DNA transfer into rat lung using this hydrodynamics-based transfection method was established. However, addition of micro babble did not enhance the efficacy of gene transfer. On the other hand, micro babble enhanced the efficacy of gene transfer via antegrade injection.
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