| Project/Area Number |
24592086
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
YOKOI KOHEI 名古屋大学, 医学(系)研究科(研究院), 教授 (60378007)
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| Co-Investigator(Kenkyū-buntansha) |
TANIGUCHI Tetsuo 名古屋大学, 医学部附属病院, 講師 (20378186)
KAWAGUCHI Koji 名古屋大学, 医学部附属病院, 病院講師 (10402611)
水野 鉄也 名古屋大学, 医学部附属病院, その他 (60528800)
宇佐美 範恭 名古屋大学, 医学部附属病院, 講師 (30378179)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 悪性胸膜中皮腫 / アスベスト / 遺伝子変異 / 癌遺伝子 / 癌抑制遺伝子 / 肺内アスベスト濃度 / がん遺伝子 / がん抑制遺伝子 |
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| Outline of Final Research Achievements |
We established some malignant pleural mesothelioma (MPM) cell lines from Japanese patients. Genome-wide array-based comparative genomic hybridization (CGH) analysis of MPMs was performed to identify regions that display DNA copy number alterations. Regions of genomic aberrations observed in > 20% of individuals were 1p36.33, 1p36.1, 1p21.3, 3p21.3, 4q22, 4q34-qter, 6q25, 9p21.3, 10p, 13q33.2, 14q32.13, 18q, and 22q of losses. The 3p21 region contained a gene, BAP1, and we further demonstrated expression of BAP1 with real-time polymerase chain reaction (PCR) analysis. The established cell line which the BAP1 protein manifestation fell, the expression of mRNA level was depression. Genomic PCR analysis detected homozygous deletion of exon 13-17 in one cell line and shorter fragment in another cell line. One cell line with BAP1 homozygous deletion shows a lack of BAP1 nuclear expression and weak cytoplasmic BAP1 staining. From these results, BAP1 might be a key gene for MPM development.
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