Involvement of E-selectin in reimplantation lung injury

• Project/Area Number: 24592100
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Keio University
• Principal Investigator: GOTO Taichiro 慶應義塾大学, 医学部, 共同研究員 (80317148)
• Co-Investigator(Kenkyū-buntansha): OHTSUKA Takashi 慶應義塾大学, 医学部, 講師 (40306717) ; OKUI Masayuki 慶應義塾大学, 医学部, 助教 (30594494) ; TAKAHASHI Yusuke 帝京大学, 医学部, 助教 (00445214) ; ANRAKU Masaki 東京大学, 医学部附属病院, 助教 (70598557)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 肺移植 / 急性肺損傷 / セレクチン / 好中球 / 動物モデル / 肺損傷 / 可溶性分画 / 肺線維症 / E-selectin / Eセレクチン

Outline of Final Research Achievements

Reimplantation lung injury is a major complication after the lung transplantation and remains yet to be solved. In this study using rat transplantation model, soluble E-selectin was found to be correlated with the lung injury score, suggesting it to be a serum marker. In the chimeric rat transplantation models, lung transplantation using the E-selectin deficient rat as the donor, caused significantly less injury, suggesting that membranous E-selectin upregulation is the trigger of acute lung injury. Serum soluble E-selectin was increased only in case wildtype lung was utilized as the donor, which suggests, soluble E-selectin could be shed and released from the membranous E-selectin. In our new lung transplantaion models, which converted the blood flow from pulmonary vein to pulmonary artery, pulmonary fibrosis resembling the interstitial pulmonary fibrosis might appear mainly in the peripheral lungs.