Development of high sensitivity detection method of CTCs in lung cancer using microfluidic chip system

• Project/Area Number: 24592108
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: OKA Soichi 産業医科大学, 医学部, 助教 (20596655)
• Co-Investigator(Kenkyū-buntansha): TANAKA Fumihiro 産業医科大学, 医学部, 教授 (10283673) ; URAMOTO Hidetaka 産業医科大学, 医学部, 准教授 (90389445) ; SO Tomoko 産業医科大学, 医学部, 講師 (00331529) ; ONAGA Takashi 富山工業技術センター中央研究所, 主幹研究員 (10416133) ; 宗 知子 産業医科大学, 医学部, 講師 (00341529)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 肺癌 / バイオマーカー / 循環腫瘍細胞 / 循環腫瘍細胞(CTC) / CTC-chip / 上皮接着分子(EpCAM) / 循環腫瘍細胞（CTC） / CellSearch / 循環血液中腫瘍細胞 / lung cancer

Outline of Final Research Achievements

Prognosis of primary lung cancer patients is not satisfactory, which is mostly caused by frequent development of distant metastasis. Although Circulating Tumor Cell (CTC) is useful for a surrogate marker of distant micro-metastasis, the CellSearch which is the only machine for CTC detection for clinical use, is insufficient sensitivity. Therefore, we developed a new microfluidic chip system of domestic, to improve sensitivity, and evaluated its basic performance. Newly developed CTC-chip not only EpCAM that have been targeted up to now showed good capture ability for other cell surface antigens. In addition, it was possible genetic analysis of the captured cells.

Research Products

• [Journal Article] Clinical Significance of IGF1R Expression in Non-Small-Cell Lung Cancer (2012)
  • Author(s): Nakagawa M, Uramoto H, Oka S (9人中3番目）
  • Journal Title: Clin Lung Cancer Volume: 2 Pages: 136-142
  • Peer Reviewed
• [Journal Article] The Expression of CD146 Predicts a Poor Overall Survival in Patients with Adenocarcinoma of the Lung (2012)
  • Author(s): Oka S, Uramoto H, Chikaishi Y, Tanaka F
  • Journal Title: Anticancer Res Volume: 32 Pages: 861-4
  • Peer Reviewed
• [Journal Article] Prognostic implications of human leukocyte antigen class I expression inpatients who underwent surgical resection for non-small-cell lung cancer. (2012)
  • Author(s): Hanagiri T, Oka S (12人中10番目）
  • Journal Title: J Surg Res Volume: nil
  • Peer Reviewed
• [Journal Article] Clinical Significance of IGF1R Expression in Non-Small-Cell Lung Cancer (2012)
  • Author(s): Nakagawa M, Uramoto H, Oka S, Chikaishi Y, Iwanami T, Shimokawa H, So T, Hanagiri T, Tanaka F
  • Journal Title: Clin Lung Cancer Volume: 13 Issue: 2 Pages: 136-142
  • DOI: 10.1016/j.cllc.2011.10.006
  • Peer Reviewed
• [Presentation] Comparison of CellSearch with polymeric microfluidic devices for CTC isolation using EpCAM-negative tumor cell lines of malignant pleural mesothelioma (2014)
  • Author(s): Yasuhiro Chikaishi1), Tomoko So1), Masaru Takenaka1), Soichi Oka1), Ayako Hirai1), Takashi Iwanami1), Kazue Yoneda1), Hidehiko Shimokawa1), Yoshika Nagata1), Hidetaka Uramoto1), Takeshi Ohnaga2), and Fumihiro Tanaka1)
  • Organizer: AACR meeting
  • Place of Presentation: San Diego CA(USA)
  • Year and Date: 2014-04-05 – 2014-04-09
• [Presentation] Development of system to detect circulating tumor cell in malignant pleural mesothelioma (2013)
  • Author(s): Yasuhiro Chikaishi, Tomoko So, Masaru Takenaka, Soichi Oka, Makoto Nakagawa, Hidehiko Shimokawa, Teruo Iwata, Hidetaka Uramoto, Takeshi Hanagiri, Fumihiro Tanaka, Takeshi Ohnaga
  • Organizer: AACR meeting
  • Place of Presentation: Washington,USA
• [Presentation] 循環腫瘍細胞（CTC）の検出方法の開発 (2013)
  • Author(s): 近石泰弘、宗 知子、米田和恵、浦本秀隆、花桐武志
  • Organizer: BioJapan2013
  • Place of Presentation: パシフィコ横浜
• [Presentation] Development of detecting systems for circulating tumor cells in malignant pleural mesothelioma patients (2013)
  • Author(s): Oka S, Chikaishi Y
  • Organizer: 第46回米国癌学会(American Association for Cancer Research, AACR)年次総会
  • Place of Presentation: Mc Cormick Place(米国・シカゴ）
• [Presentation] Circulating tumor cell (CTC) as a significant clinical marker in epithelioid-type malignant pleural mesothelioma (2012)
  • Author(s): Yoneda K, Tanaka F, Chikaishi, Oka S
  • Organizer: 第48回米国癌治療学会(American Society of Clinical Oncology, ASCO)年次総会
  • Place of Presentation: Cormick Place(米国・シカゴ）