| Project/Area Number |
24592126
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kobe University |
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Principal Investigator |
HOSODA Kohkichi 神戸大学, 医学(系)研究科(研究院), 准教授 (90403261)
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| Co-Investigator(Kenkyū-buntansha) |
KOYAMA Junji 神戸大学, 大学院医学研究科, 研究員 (00594508)
KOHMURA Eiji 神戸大学, 大学院医学研究科, 教授 (30225388)
UCHIHASHI Yoshito 神戸大学, 大学院医学研究科, 研究員 (80605999)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIDA Masaru 神戸大学, 大学院医学研究科, 准教授 (00419475)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | metabolomics / cerebral ischemia / metabolic pathway / MCA occlusion / RT-PCA / microarray / 脳虚血 / 脳梗塞 / メタボロミクス / 頚動脈狭窄症 / 一過性脳虚血 |
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| Outline of Final Research Achievements |
We created cerebral infarction by use of rat middle cerebral artery occlusion and sampled the cerebral cortex, which was analyzed by metabolome analysis with the use of gas-chromatography/mass-spectrometry. The result implicated that the tricarboxylic acid cycle (TCA) cycle was stopped and that the anaplerotic responses such as malate-aspartate shuttle and purine nucleotide cycle were upregulated and that the pentose phosphate pathway (PPP) was also upregulated. Consequently, during ultra-acute stage of cerebral ischemia, it is most likely that TCA cycle was stopped due to ATP depletion and the anaplerotic responses was upregulated and PPP was upregulated to increase NADPH to deal with the reactive oxygen species.
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