In vivo PET and optical imaging of neurogenesis of neuronal circuit

• Project/Area Number: 24592155
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: INAJI Motoki 東京医科歯科大学, 医学部附属病院, 講師 (00422486)
• Co-Investigator(Renkei-kenkyūsha): HIGUCHI Makoto 独立行政法人放射線医学総合研究所分子神経イメージンググループ, グループリーダー (10373359) ; MAEDA Jun 独立行政法人放射線医学総合研究所分子神経イメージンググループ, 主任研究員 (30415426) ; SATO Katsushige 駒沢女子大学, 人間健康学部, 教授 (80291342)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: PET / 光学計測 / 神経移植

Outline of Final Research Achievements

1. Optical recording method have demonstrated the development of cortico-striatum circuit in the slice of the rat brain. It also showed the electro-physiolosical connection from transplanted cells in the slice of PD model rats. We also evaluated new voltage-sensitive fluorescence dyes for monitoring neuronal activity in the embryonic central nervous system.2. We demonstrated cell proliferation precisely in brain tumor patients using 11C-4DST-PET image. It can also visualize the neurogenesis of transplanted cells. 3.PET imaging of transplanted PD model rats showed the time course of expression of dopamine transporter and normalization of dopamine D2 receptors binding.

Research Products

• [Journal Article] Clinical benefit of 11C methionine PET imaging as a planning modality for radiosurgery of previously irradiated recurrent brain metastases (2014)
  • Author(s): Momose, T. Nariai, T. Kawabe, T. Inaji, M. Tanaka, Y. Watanabe, S. Maehara, T. Oda, K. Ishii, K. Ishiwata, K. Yamamoto, M.
  • Journal Title: Clin Nucl Med Volume: 39 Issue: 11 Pages: 939-943
  • DOI: 10.1097/rlu.0000000000000561
  • Peer Reviewed / Open Access
• [Journal Article] PET, SPECT, and MEG in the diagnosis of epilepsy (2014)
  • Author(s): Inaji M, Maehara T
  • Journal Title: Nihon Rinsho Volume: 72 Pages: 827-33
• [Journal Article] Practical Clinical Use of Dynamic Susceptibility Contrast Magnetic Resonance Imaging (DSC-MRI) for the Surgical Treatment of Moyamoya Disease. (2014)
  • Author(s): 5. Ishii Y, Nariai T, Tanaka Y, Mukawa M, Inaji M, Maehara T, Ohno K.
  • Journal Title: Neurosurgery Volume: 74（3） Issue: 3 Pages: 302-309
  • DOI: 10.1227/neu.0000000000000266
  • Peer Reviewed
• [Journal Article] Surgical effects of focus resection for patients with intractable epilepsy. (2013)
  • Author(s): Maehara T, Inaji M, Matsuura M.
  • Journal Title: Neurol Med Chir (Tokyo) Volume: 53 Pages: 281-6
  • NAID: 10031158753
  • Peer Reviewed
• [Journal Article] Evaluation of voltage-sensitive fluorescence dyes for monitoring neuronal activity in the embryonic central nervous system. (2013)
  • Author(s): Mullah, S. H.-E.-R., Komuro, R., Yan, P., Hayashi, S., Inaji, M., Momose- Sato, Y., Loew, L. M. and Sato, K.
  • Journal Title: The Journal of Membrane Biology Volume: 246 Issue: 9 Pages: 679-688
  • DOI: 10.1007/s00232-013-9584-1
  • Peer Reviewed
• [Journal Article] PET molecular imaging to investigate higher brain dysfunction in patients with neurotrauma. (2013)
  • Author(s): Nariai T, Inaji M, Tanaka Y, Hiura M, Hosoda C, Ishii K, Ohno K.
  • Journal Title: Acta Neurochir Suppl Volume: 118 Pages: 251-4
  • DOI: 10.1007/978-3-7091-1434-6_47
  • ISBN: 9783709114339, 9783709114346
  • Peer Reviewed
• [Journal Article] A selective adenosine A2A receptor antagonist ameliorated hyperlocomotion in an animal model of lateral fluid percussion brain injury. (2013)
  • Author(s): Mullah SH, Inaji M, Nariai T, Ishibashi S, Ohno K.
  • Journal Title: Acta Neurochir Suppl Volume: 118 Pages: 89-92
  • DOI: 10.1007/978-3-7091-1434-6_15
  • ISBN: 9783709114339, 9783709114346
  • Peer Reviewed
• [Journal Article] Post-operative mismatch negativity recovery in a temporal lobe epilepsy patient with cavernous angioma. (2013)
  • Author(s): Hara K, Maehara T, Miyajima M, Ohta K, Iino H, Inaji M, Matsuda A, Matsushima E, Hara M, Matsuura M.
  • Journal Title: Clin Neurol Neurosurg Volume: 115 Issue: 6 Pages: 756-9
  • DOI: 10.1016/j.clineuro.2012.06.037
  • Peer Reviewed
• [Journal Article] A Selective Adenosine A2A Receptor Antagonist Ameliorated Hyperlocomotion in an Animal Model of Lateral Fluid Percussion Brain Injury (2013)
  • Author(s): Saad Habib-E-Rasul Mullah MD, PhD, Motoki Inaji MD, PhD, Tadashi Nariai MD, PhD, Satoru Ishibashi MD, PhD, Kikuo Ohno MD, PhD
  • Journal Title: Acta Neurochirurgica Supplement Volume: 118 Pages: 89-92
  • Peer Reviewed
• [Journal Article] Optical analysis of developmental changes in synaptic projection in the neonatal rat cortical projection (2012)
  • Author(s): SaadHabib-E-Rasul, Motoki Inaji, Tadashi Nariai, Yoko Sato, Katsushige Sato, Kikuo Ohno
  • Journal Title: Neuroscience Volume: 201 Pages: 1831-1839
  • Peer Reviewed
• [Journal Article] Long-term follow-up of surgically treated juvenile patients with Moyamoya disease. (2012)
  • Author(s): Mukawa M, Nariai T, Matsushima Y, Tanaka Y, Inaji M, Maehara T, Aoyagi M, Ohno K.
  • Journal Title: J Neurosurg Pediatr Volume: 10 Pages: 451-6
  • Peer Reviewed
• [Presentation] The effects of reports of car accidents caused by persons with epilepsy on its stigma in Japan. (2014)
  • Author(s): Hara K, Tanaka S, Hirose Y, Tohma S, Tabata S, Matsuura M, Ohta K, Inaji M, Hara M, Maehara T, Sumi Y:
  • Organizer: annual meeting of American Epilepsy Society
  • Place of Presentation: Seattle,USA
  • Year and Date: 2014-12-05 – 2014-12-09
• [Presentation] 側頭葉てんかん術中脳波における海馬HFO測定 (2014)
  • Author(s): 稲次基希、原 恵子、田端 梓、前原健寿
  • Organizer: 第44回日本臨床神経生理学会学術大会
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 2014-11-19 – 2014-11-21
• [Presentation] Monitoring of biological response to bevacizumab in malignant glioma patients based on L-[methyl-11C] methionine-positron emission tomography. (2014)
  • Author(s): Tamura K, Inaji M, Nariai T, Tamaki M, Hirai S, Saigusa K, Tanaka Y, Maehara T
  • Organizer: Annual Scientific Meeting of the Society for Neuro-Oncology
  • Place of Presentation: Miami,Turky
  • Year and Date: 2014-11-13 – 2014-11-16
• [Presentation] Malignant glioma adjuvant therapyにおけるPET clinical useの有用性 (2014)
  • Author(s): 稲次基希、成相 直、林志保里、田中洋次、織田圭一、坂田宗之、豊原 潤、石渡喜一、石井賢二、前原健寿
  • Organizer: 第54回日本核医学技術学会総会学術大会
  • Place of Presentation: 国際会議場、大阪
  • Year and Date: 2014-11-06 – 2014-11-08
• [Presentation] Treatment of Adult patients with Moyamoya Disease Based On Hemodynamic Measurement with perfusion MRI and PET and indirect bypass procedures. (2014)
  • Author(s): Nariai T, Inaji M, Hayashi S, Ishii Y, Mukawa M, Tanaka Y, Maehara T, Ishiwata K, Ishii K
  • Organizer: World Stroke Congress
  • Place of Presentation: Istanbuhl,Turky
  • Year and Date: 2014-10-22 – 2014-10-25
• [Presentation] Treatment of juvenile patients with moyamoya disease based on non-invasive measurement of cerebral perfusion with MRI (2014)
  • Author(s): Nariai T, Tanaka Y, Ishii Y, Hara S-M, Ueda Y, Mukawa M, Inaji M, Maehara T
  • Organizer: World Stroke Congress
  • Place of Presentation: Istanbuhl
  • Year and Date: 2014-10-22 – 2014-10-25
• [Presentation] Significance of PET Adenosine A1 receptor imaging with MPDX PET to investigate on the mechanism to protect the secondary brain dysfunction in temporal lobe epilepsy (2014)
  • Author(s): Hayashi S, Inaji M, Nariai T, Sakata M, Ishii K, Hara K, Maehara T
  • Organizer: Asian Epilepsy Surgery Congress
  • Place of Presentation: 都市センターホテル、東京
  • Year and Date: 2014-10-14 – 2014-10-16
• [Presentation] Postoperative psychiatric condition in intractable epilepsy patients. (2014)
  • Author(s): Inaji M, Maehara T, Nariai T, Hayashi S, Hara K, Sakata M, Ishiwata K, Ishii K
  • Organizer: Asian Epilepsy Surgery Congress
  • Place of Presentation: 都市センターホテル、東京
  • Year and Date: 2014-10-14 – 2014-10-16
• [Presentation] Our experiences of hippocampal transection for various types of TLE (2014)
  • Author(s): Maehara T, Inaji M, Hayashi S, Orihara A, Tabata A, Hara K, Matsuura M
  • Organizer: Asian Epilepsy Surgery Congress
  • Place of Presentation: 都市センターホテル、東京
  • Year and Date: 2014-10-14 – 2014-10-16
• [Presentation] Usefulness of ictal HFO analysis and hippocampal transection for patients with MRI-negative neocortical epilepsy (2014)
  • Author(s): Maehara T, Inaji M, Hayashi S, Orihara A, Tabata A, Hara K
  • Organizer: Asian Epilepsy Surgery Congress
  • Place of Presentation: 都市センターホテル、東京
  • Year and Date: 2014-10-14 – 2014-10-16
• [Presentation] Surgical outcome for temporal lobe epilepsy due to vascular malformation (2014)
  • Author(s): Orihara A, Inaji M, Hara K, Matsuura M, Maehara T
  • Organizer: Asian Epilepsy Surgery Congress
  • Place of Presentation: 都市センターホテル、東京
  • Year and Date: 2014-10-14 – 2014-10-16
• [Presentation] 頭部外傷におけるアデノシン受容体の治療、診断ターゲットとしての可能性 (2014)
  • Author(s): 稲次基希、成相 直、Mullah Saad、田中洋次、林志保里、坂田宗之、織田圭一、石渡喜一、石井賢二、前原健寿
  • Organizer: 日本脳神経外科学会第73回学術総会
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 2014-10-09 – 2014-10-11
• [Presentation] てんかん患者における神経心理検査とFDGPETの相関 (2014)
  • Author(s): 稲次基希、林志保里、成相 直、原 恵子、松浦雅人、前原健寿
  • Organizer: 第48回日本てんかん学会学術集会
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 2014-10-02 – 2014-10-03
  • Invited
• [Presentation] Roles of Methionine PET in Gamma Knife Radiosurgery for Malignant Glioma. (2014)
  • Author(s): Nariai T, Yamamoto M, Momose T, Inaji M, Tanaka Y, Kawabe T, Nagatomo Y, Barfod B, Ishiwata K, Ishii K
  • Organizer: 17th Leksell GK Society Meeting
  • Place of Presentation: New York,USA
  • Year and Date: 2014-05-11 – 2014-05-15
• [Presentation] Imaging of DNA synthesis rate of malignant brain tumor with a newly developed PET molecular imaging probe 11C-4DST. (2013)
  • Author(s): Hayashi, S., Inaji, M., Nariai, T., Tanaka, Y., Maehara, T., Oda, K., Sakata, M., Toyohara, J., Ishiwata K. and Ishii, K.
  • Organizer: 15th WFNS World Congress of Neurosurgery
  • Place of Presentation: Seoul,Korea
• [Presentation] Validation of MRI T1/T2 mismatch method for accurate differentiation between radiation injury and recurrence of metastatic brain tumor after gamma knife radiosurgery. –Comparison with methionine PET – (2013)
  • Author(s): Inaji M, Yamamoto M, Nariai T, Tanaka Y, Urakawa Y, Ishiwata K, Ishii K, Maehara T
  • Organizer: 11th International Stereotactic Radiosurgery Society Congress,
  • Place of Presentation: Toronto,Canada
• [Presentation] 11C-4DST PETのGlioma悪性度診断における有用性と妥当性の検証 (2013)
  • Author(s): 稲次基希、成相直、林志保里、田中洋次、織田圭一、坂田宗之、石渡喜一、豊原潤、石井賢二、前原健寿
  • Organizer: 第53回日本核医学会学術総会,
  • Place of Presentation: 福岡（福岡国際会議場）
• [Presentation] 新生ラット皮質線条体投射におけるLTPの個体発生：光学的イメージングによる解析 (2013)
  • Author(s): 佐藤勝重、Mullah Habib-E-Rasul Saad、稲次基希、成相直、佐藤容子、大野喜久 郎
  • Organizer: 第90回日本生理学会
  • Place of Presentation: 東京
• [Presentation] 新生ラット皮質線条体投射におけるシナプス長期増強の個体発生学的変化の光学的解 析 (2012)
  • Author(s): 佐藤勝重、Mullah Habib-E-Rasul Saad、稲次基希、成相直、佐藤容子、大野喜久郎
  • Organizer: 第35回日本神経科学学会
  • Place of Presentation: 名古屋
• [Presentation] Developmental changes in the LTP in the neonatal rat corticostriatal projection: Optical imaging with a voltage-sensitive dye. (2012)
  • Author(s): Mullah, S. H.-E.-R. , Inaji, M., Nariai, T., Urmy, J., Momose-Sato, Y., Ohno, K. and Sato, K.
  • Organizer: Society for Neuroscience 42nd Annual Meeting
  • Place of Presentation: New Orleans
• [Presentation] 11C-4DSTを用いたDNA合成能イメージングによる悪性脳腫瘍診断 (2012)
  • Author(s): 稲次基希、成相直、田中洋次、青柳傑、坂田宗之、豊原潤、石渡喜一、石井賢二
  • Organizer: 第13回日本分子脳神経外科学会総会
  • Place of Presentation: 熊本
• [Presentation] 11C-4DSTPETによる悪性脳腫瘍DNA合成能イメージング (2012)
  • Author(s): 稲次基希、成相直、田中洋次、青柳傑、坂田宗之、豊原潤、石渡喜一、石井賢二
  • Organizer: 日本脳神経外科学会総会
  • Place of Presentation: 大阪
• [Book] Tumors of the central nervous system (2014)
  • Author(s): Nariai T, Inaji M, Sakata M, Toyohara J
  • Total Pages: 8
  • Publisher: Springer
• [Book] アクチュアル 脳・神経疾患の臨床 (2014)
  • Author(s): 稲次基希、大野喜久郎
  • Total Pages: 7
  • Publisher: 中山書店
• [Book] Medicina (2014)
  • Author(s): 稲次基希、前原健寿.
  • Total Pages: 6
  • Publisher: 医学書院
• [Book] 再生医療における臨床研究と製品開発 (2013)
  • Author(s): 稲次基希
  • Total Pages: 6
  • Publisher: 技術情報協会