Role of sonic hedgehog signaling in migration of malignant glioma

• Project/Area Number: 24592167
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Kagoshima University
• Principal Investigator: UCHIDA Hiroyuki 鹿児島大学, 医学部・歯学部附属病院, 特任助教 (80404482)
• Co-Investigator(Kenkyū-buntansha): HIRANO Hirofumi 鹿児島大学, 医学部・歯学部附属病院, 講師 (00264416) ; YUNOUE Shunji 鹿児島大学, 医歯(薬)学総合研究科, 客員研究員 (20404478) ; ARITA Kazunori 鹿児島大学, 医歯(薬)学総合研究科, 教授 (90212646)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: Glioma / Cancer stem cell / CD133 / migration / Sonic hedgehog / Migration / glioma / cancer stem cell / sonic hedgehog

Outline of Final Research Achievements

This study was intended to establishment of the glioma stem cell line and evaluate the influence of SHH pathway to migration of the glioma stem cells. CD133-positive cells in malignant glioma tissue were extracted using an automagnetic cell separator and tumor spheres were obtained. Gene expression of SHH pathway was detected in almost cases of malignant glioma. It was suggested that SHH participated in maintenance of stemness and migration. In adherent cells confirmed in a process of subculture, CD133-positive cells existed more than in normal malignant glioma cell lines.

Research Products

• [Journal Article] Improvement in treatment results of glioblastoma over the last three decades and beneficial factors (2015)
  • Author(s): Kawano H, Hierano H, Yonezawa H, Yatsushiro K, Ogita M, Hiraki Y, Uchida H, Habu M, Fujio S, Oyoshi T, Bakhtiar Y, Sugata S, Yamahata H, Hanaya R, Tokimura H, Arita K
  • Journal Title: British Journal of Neurosurgery Volume: 29 Issue: 2 Pages: 206-212
  • DOI: 10.3109/02688697.2014.967750