Investigation for abnormal distribution of SV2A and disorder of synaptic vesicle release in refractory epilepsy

• Project/Area Number: 24592169
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Kagoshima University
• Principal Investigator: HANAYA Ryosuke 鹿児島大学, 医学部・歯学部附属病院, 講師 (60304424)
• Co-Investigator(Kenkyū-buntansha): YUNOUE Syunji 鹿児島大学, 医歯(薬)学総合研究科, 研究員 (20404478) ; SUGATA Sei 鹿児島大学, 医歯(薬)学総合研究科, 研究員 (70437961) ; ARITA Kazunori 鹿児島大学, 医歯(薬)学総合研究科, 教授 (90212646)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: てんかん原性 / シナプス小胞蛋白 / SVA2 / Synaptotagmin-1 / FDG-PET / てんかん / SV2A / SNARE関連蛋白 / sinaptotagmin-1 / SNARE蛋白

Outline of Final Research Achievements

We immunohistochemically evaluated the role of synaptic vesicle protein, SV2A about the expression of epilepsy using specimens of surgically treated-patients. SV2A decreased in the wide area of the brain, and the decrease of synaptotagmin-1 localized around epilepsy focus, whereas SNARE-related protein disorder would participate in the formation of epileptogenicity. Hypometabolism of FDG-PET, which indicated brain dysfunction overlapped in the decease of SV2A. SV2A mediated synaptic vesicle-releasing mechanism would be disturbed in dysfunctional brain around epileptogenic area, it was suggested that.

Research Products

• [Presentation] 結節性硬化症に伴うてんかんにおける11C-Methionine PETによるてんかん原性結節の同定 (2014)
  • Author(s): 花谷亮典 大坪俊昭 樫田祐美 細山浩史 菅田真生 丸山慎介 飯田幸治 中村克己 藤元登四郎 有田和徳
  • Organizer: 第35回日本てんかん外科学会
  • Place of Presentation: 大阪
• [Presentation] 南九州におけるてんかん診療連携の試み (2014)
  • Author(s): 花谷亮典
  • Organizer: 第4回Kyushu Neuroscience Conference
  • Place of Presentation: 福岡
  • Invited
• [Presentation] The role of 11C-Methionine positron emission tomography in evaluation for epileptogenic region (2013)
  • Author(s): Hanaya R, Otsubo T, Kashida Y, Hosoyama H, Sugata S, Nakamura K, Fujimoto T, Iida K, Arita K.
  • Organizer: 36th International Epilepsy Congress
  • Place of Presentation: Montreal, Canada
• [Presentation] Neuroprotective effect of levetiracetam on hippocampal sclerosis-like change in spontaneously epileptic rats (2013)
  • Author(s): Sugata S, Hanaya R, Serikawa T, Kurisu K, Arita K, Sasa M
  • Organizer: 30th International Epilepsy Congress
  • Place of Presentation: Montreal, Canada
• [Presentation] The experience of image guided operation oriented by using intraoperative MRI in epilepsy surgery (2013)
  • Author(s): Kashida Y, Hanaya R, Otubo T, Hosoyama H, Sugata S, Bakhtiar Y, Nakamura K, Fujimoto T, Iida K, Arita K.
  • Organizer: 30th International Epilepsy Congress
  • Place of Presentation: Montreal, Canada
• [Presentation] 難治性新皮質てんかんにおける発作間歇期FDG-PET集積低下とSV2A発現低下との関連性
  • Author(s): 花谷亮典, 細山浩史, 菅田真生, 大坪俊昭, 藤元登四郎, 飯田幸治, 有田和徳
  • Organizer: 第46回日本てんかん学会
  • Place of Presentation: 都市センターホテル
• [Presentation] てんかん原性領域を超えるFDG-PET低集積部位の評価
  • Author(s): 花谷亮典, 大坪俊昭, 細山浩史, 菅田真生, 新里能成, 湯之上俊二, 中村克巳, 飯田幸治, 藤元登四郎, 有田和徳
  • Organizer: 第71回日本脳神経外科学会総会
  • Place of Presentation: 大阪国際会議場