Mre11-Rad50-Nbs1 complex inhibitor Mirin enhances radiosensitivity in human glioblastoma cells
Project/Area Number |
24592171
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Cerebral neurosurgery
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Research Institution | Saitama Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
KAWATA Tetsuya 慶應義塾大学, 医学部, 講師 (60234077)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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Keywords | glioma / radiation therapy / radiosensitivity / Mre11-Rad50-Nbs1 / DNA damage / 放射線治療 / DNA 損傷 / 放射線増感 / MER11-RAD50-NBS1 / グリオーマ / DNA損傷 / MRE11-RAD50-NBS1 / DNA損傷 |
Outline of Final Research Achievements |
Radiation therapy plays a central part in the treatment of glioblastoma, however, it is not curative due to the high tumor radioresistance. The Mre11, Rad 50 and Nbs1 proteins form a complex (MRN) that has a critical role in DNA damage detection and signaling. Here, we investigated the effects of the MRN complex inhibitor, Mirin, on radiation response of human glioma cells. Glioma cell lines were irradiated with and without Mirin and then clonogenicity, apoptosis, and cell cycle change were examined. Glioblastoma cells pretreated with Mirin demonstrated an enhanced sensitivity to radiation. FACS analysis revealed that the combination of Mirin and radiation treatments significantly increased the G2/M fraction of the glioma cells. Mirin enhanced radiation-induced apoptotic cell death and mitotic catastrophe. These results indicate that Mirin can effectively enhance glioma cell radiosensitivity. It suggests that Mirin is a potent radiosensitizer for treating glioma cells.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] A Multicenter Phase I/II Study of the BCNU Implant (Gliadel® Wafer) for Japanese Patients with Malignant Gliomas.2014
Author(s)
Aoki T, Nishikawa R, Sugiyama K, Nonoguchi N, Kawabata N, Mishima K, Adachi JI, Kurisu K, Yamasaki F, Tominaga T, Kumabe T, Ueki K, Higuchi F, Yamamoto T, Ishikawa E, Takeshima H, Yamashita S, Arita K, Hirano H, Yamada S, Matsutani M.
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Journal Title
Neurol Med Chir (Tokyo)
Volume: 54
Pages: 290-301
NAID
Related Report
Peer Reviewed
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[Journal Article] Immunochemotherapy with rituximab and methotorexate with deferred radiotherapy for newly diagnosed primary CNS lymphoma (PCNSL) in the elderly2013
Author(s)
Mishima K, Adachi J, Suzuki T, Uchida E, Yanagawa T, Watanabe Y, Fukuoka K, Yanagisawa T, Wakiya K, Fujimaki T, Nishikawa R.
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Journal Title
Neuro-Oncology
Volume: 15
NAID
Related Report
Peer Reviewed
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[Journal Article] Randomized trial of chemoradiotherapy and adjuvant chemotherapy with nimustine versus nimustine plus procarbazine for newly diagnosed anaplastic astrocytoma and glioblastoma .2013
Author(s)
Shibui S, Narita Y, Mizusawa J, Beppu T, Ogasawara K, Sawamura Y, Kobayashi H, Nishikawa R, Mishima K, Muragaki Y, Maruyama T, Kuratsu J, Nomura K etl .
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Journal Title
Cancer Chemother Pharmacol.
Volume: 71(2)
Issue: 2
Pages: 511-21
DOI
Related Report
Peer Reviewed
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[Journal Article] Immunohistochemical analysis-based proteomic subclassification of newly diagnosed glioblastomas with integrated genomic and epigenetic profiles2012
Author(s)
Motomura K, Natsume A, Watanabe R, Ito I, Kato Y, Momota H, Nishikawa R, Mishima K, Nakasu Y, Abe T, Namba H, Nakazato Y, Tashiro H, Takeuchi I, Mori T, Wakabayash T.
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Journal Title
Cancer Sci.
Volume: 103(10)
Issue: 10
Pages: 1871-1879
DOI
Related Report
Peer Reviewed
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[Presentation] Immunochemotherapy with rituximab and methotorexate with deferred radiotherapy for newly diagnosed primary CNS lymphoma (PCNSL) in the elderly2013
Author(s)
Mishima K, Adachi J, Suzuki T, Uchida E, Yanagawa T, Watanabe Y, Fukuoka K, Yanagisawa T, Wakiya K, Fujimaki T, Nishikawa R.
Organizer
4th Quadrennial Meeting of the World Federation of Neuro-Oncology
Place of Presentation
San Francisco, 米国
Related Report
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