Role of synaptic vesicle 2A protein to obtain epileptogenesis through neurogenesis in hippocampus
| Project/Area Number |
24592173
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Madoka 順天堂大学, 医学部, 准教授 (50317450)
ARAI Hajime 順天堂大学, 医学部, 教授 (70167229)
NISHIMURA Kinya 順天堂大学, 医学部, 教授 (80164581)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 神経細胞新生 / 海馬 / てんかん / SV2A / 神経新生 / パッチクランプ / Levetiracetam / levetiracetam |
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| Outline of Final Research Achievements |
We investigate our hypothesis that inhibition of SV2A can stabilize the neurogenesis and consequently prevent the epileptogenesis. We evaluated an appearance and electrical characteristics of newborn cells in the hippocampus after initial seizure insult in this study. We counted and observed its morphology of newborn cells among Levetiracetam (LEV) (SV2A stabilizer) treatment and non-treatment groups. The number of newborn neurons in the LEV group was significantly less than that of the non-treatment group. Furthermore, LEV made normalized the cell appearance. In the results of patch-clump method, early appeared newborn neurons were immature with easily excitable characteristics, however survived neuron for 28 days from initial seizure insult showed the same electrical characteristics as that of matured neuron. From these results, stabilization of SV2A after an initial incentive has a possibility to prevent inducing epileptogenicity through neurogenesis.
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Report
(4 results)
Research Products
(14 results)
