A novel chemotherapy of malignant glioma by concomitant use of inhibitors for angiogenetic factor and hypoxia-inducible factor

• Project/Area Number: 24592178
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Fujita Health University
• Principal Investigator: HAYASHI Takuro 藤田保健衛生大学, 医学部, 客員講師 (40296611)
• Co-Investigator(Kenkyū-buntansha): HIROSE Yuichi 藤田保健衛生大学, 医学部, 教授 (60218849) ; ITO Keisuke 藤田保健衛生大学, 医学部, 助教 (70622934)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: malignant glioma / bevacizumab / rapamycin / angiogenesis / hypoxia / xenograft / 悪性グリオーマ / ベバシズマブ / ラパマイシン / 移植 / 低酸素 / 血管新生 / HIF1α / MRI / グリオーマ

Outline of Final Research Achievements

Using human glioma cell line U251, biological responses of glioma cells to combination usage of angiogenesis inhibitor (bevacizumab) and chemotherapeutic agent (rapamycin) was investigated in vitro and in vivo. The result of this study suggested that the growth of glioma might be inhibited by rapamycin,which blocked the secondary angiogenesis, resulted from hypoxic state by bevacizumab. Thus, this type of concomitant therapy could be one of the novel approaches of malignant glioma.

Research Products

• [Journal Article] Nestin overexpression precedes caspase-3 upregulation in rats exposed to controlled cortical impact traumatic brain injury. (2012)
  • Author(s): Kaneko Y, Tajiri N, Yu S, Hayashi T, Stahl CE, Bae E, Mestre H, Franzese N, 他（21人中4番目）
  • Journal Title: Cell Med. Volume: 4(2) Pages: 55-63
  • Peer Reviewed
• [Journal Article] The role of catecholamines in the pathogenesis of neurogenic pulmonary edema associated with subarachnoid hemorrhage. (2012)
  • Author(s): Inamasu J, Sugimoto K, Yamada Y, Ganaha T, Ito K, Watabe T, Hayashi T, Kato Y, Ozaki Y, Hirose Y.
  • Journal Title: Acta Neurochir (Wien) Volume: Dec;154(12) Issue: 12 Pages: 2179-84
  • DOI: 10.1007/s00701-012-1515-x
  • Peer Reviewed
• [Journal Article] Clinical, Histological, and Genetic Features of Fourth Ventricle Ependymoma in the Elderly (2012)
  • Author(s): Hayashi T, Inamasu J, Kanai R, Sasaki H, Shinoda J, Hirose Y
  • Journal Title: Neurologia medico-chirurgica Volume: 52 Issue: 8 Pages: 611-616
  • DOI: 10.2176/nmc.52.611
  • NAID: 10030694858
  • ISSN: 0470-8105, 1349-8029
  • Peer Reviewed
• [Journal Article] [Effect of levetiracetam on intractable secondarily generalized seizure and depression]. (2012)
  • Author(s): Hayashi T, Inoue K, Shinoda J.
  • Journal Title: Brain Nerve. Volume: Mar;64(3) Pages: 303-7
  • Peer Reviewed
• [Presentation] 大脳半球梗塞に対する開頭減圧術後の死亡症例に関する検討 (2013)
  • Author(s): 林 拓郎、稲桝丈司、井水秀栄、我那覇司、山田康博、垣内孝史、早川基治、渡部剛也、加藤庸子、廣瀬雄一
  • Organizer: 第18回日本脳神経外科救急学会
  • Place of Presentation: 弘前
• [Presentation] グリオーマ細胞移植マウスにおけるCdk阻害剤併用によるテモゾロミド増強効果に関する免疫組織学的および放射線学的検討 (2012)
  • Author(s): 林 拓郎、長久伸也、西山悠也、安達一英、田之上俊介、伊藤圭介、長谷川光広、廣瀬雄一
  • Organizer: 日本脳神経外科学会 第71回学術総会
  • Place of Presentation: 大阪
• [Presentation] グリオーマ細胞移植マウスにおけるCdk阻害剤併用によるテモゾロミドの増感効果 (2012)
  • Author(s): 林 拓郎、長久伸也、西山悠也、中江俊介、田之上俊介、安達一英、長谷川光広、廣瀬雄一
  • Organizer: 第30回日本脳腫瘍学会学術集会
  • Place of Presentation: 広島
• [Presentation] 神経血管減圧術でのteflon-induced granulomaによる三叉神経痛の手術所見～一例報告～
  • Author(s): 林 拓郎、長谷川光広、長久伸也、吉田耕一郎、西山悠也、安達一英、廣瀬雄一
  • Organizer: 第14回名古屋脳神経外科領域痛みの研究会
  • Place of Presentation: 名古屋