| Project/Area Number |
24592186
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
MURATA ATSUSHI 千葉大学, 医学部附属病院, 准教授 (20344997)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Masashi 筑波大学, 医学医療系, 教授 (50281712)
大河 昭彦 千葉大学, 医学(系)研究科(研究院), 講師 (30312945)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
|---|
| Keywords | neuropathic pain / CCI / mRNA / microarray / Noggin / Alzet pump / rats / astrocytes / 神経障害性疼痛 / 坐骨神経結紮モデル / アロジニア / BMP4 / くも膜下腔 / Western blot |
|---|
| Outline of Final Research Achievements |
We find that Noggin mRNA is down-regulated in the lumber enlargement two weeks after Chronic Constriction injury (CCI) in the rats by a Microarray and RT-PCR study. In the present study, we injected Noggin protein intrathecally using Alzet-mini pump for CCI model and see the neuropathic pain state using von Frey test for five weeks. Two weeks after CCI, Alzet-mini pump (14 days) with Noggin was inserted intrathecally. Rats whose Noggin concentration was 2microg/ml showed significantly less hyper-sensation compared with control.
We next compared intrathecal Noggin injection (2microg/ml) with BMP4 (2microg/ml), or BMP4 (4microg/ml) +Noggin (4microg/ml) injection, and compared statistically with control. Noggin injection only showed statistically significant less hyper-sensation than others. Fluorescent immunohistochemistry showed Noggin injection partially decreased astrocytes accumulation in the dorsal horn compared with PBS control one week after intrathecal Noggin injection.
|
