Basic research of anti-NGF inhibitor for chronic musculoskeletal pain

• Project/Area Number: 24592187
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Chiba University
• Principal Investigator: KAZUHISA Takahashi 千葉大学, 医学(系)研究科(研究院), 教授 (20179477)
• Co-Investigator(Kenkyū-buntansha): OHTORI Seiji 千葉大学, 大学院医学研究院, 講師 (40361430) ; INOUE Gen 北里大学, 医学部, 准教授 (80594209) ; ORITA Sumihisa 千葉大学医学部付属病院, 材料部, 助教 (60638310)
• Co-Investigator(Renkei-kenkyūsha): MIYAGI Masayuki 北里大学, 医学部, 助教 (90627556) ; SUZUKI Miyako 千葉大学, 予防医学センター, 助教 (70734242) ; TOYONE Tomoaki 帝京大学, 溝口病院整形外科, 教授 (10407918) ; OZAWA Tomoyuki 帝京大学, 溝口病院整形外科, 講師 (60375706) ; AOKI Yasuchika 千葉大学, 大学院医学研究院, 特任教授 (70584001)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 抗NGF療法 / CGRP / ラット尾椎椎間板圧迫モデル / 椎間板性腰痛 / 抗サイトカイン療法 / MIA / 変形性膝関節症 / 神経栄養因子 / 椎間板性疼痛 / 頸痛症 / 慢性運動器疼痛 / 神経成長因子

Outline of Final Research Achievements

Create a rat caudal vertebrae intervertebral disc pressure model, we investigated the expression of calcitonin gene-related peptide (CGRP: neuropeptide involved in the inflammatory disease) in a cell body of dorsal root ganglia (DRG)to dominate the sensory nerve of the intervertebral disc. Intradiscal administration of the anti-NGF neutralizing antibodies suppressed the increased expression of CGRP of dominant sensory nerve (DRG). For the discogenic low back pain, anti-NGF therapy suggesting the potential to be effective .

Research Products

• [Journal Article] Efficacy of Anti-Nerve Growth Factor Therapy for Discogenic Neck Pain in Rats. (2014)
  • Author(s): Sainoh T, Sakuma Y, Miyagi M, Orita S, Yamauchi K, Inoue G, Kamoda H, Ishikawa T, Suzuki M, Kubota G, Oikawa Y, Inage K, Sato J, Nakamura J, Aoki Y, Takaso M, Toyone T, Takahashi K, Ohtori S.
  • Journal Title: Spine Volume: 未定
  • Peer Reviewed