Pathology & treatment of spinal cord injury
Project/Area Number |
24592192
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Orthopaedic surgery
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Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
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Research Collaborator |
HANADA Mitsuru 浜松医科大学, 医学部, 医局員
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 脊髄損傷 / 質量分析顕微鏡法 / MALDI / プロスタグランディン / LC-MS/MS / IL-6受容体抗体 / ドコサヘキサエン酸 / 筋萎縮性側索硬化症 / フォスファチジルコリン |
Outline of Final Research Achievements |
Imaging mass spectrometry was used to visualize twelve molecular species of phosphatidylcholine (PC) on thin slices of spinal cord with spinal cord injury (SCI). Unique distribution patterns were observed for phospholipids with different fatty acid compositions, and distinct dynamic changes were seen in both their amounts and their distributions in tissue as tissue damage resulting from SCI progressed. In particular, PCs containing docosahexaenoic acid localized to the large nucleus in the anterior horn region at one day post-SCI and rapidly decreased thereafter. PC imaging revealed that the anti-IL-6 receptor antibody MR16-1 that has been shown to suppress inflammation after SCI was able to prevent the reduction of docosahexaenoic acid-containing PC in comparison with the control group at the injury site as early as 7 days post-SCI.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Reductions of docosahexaenoic acid-containing phosphatidylcholine levels in the anterior horn of an ALS mouse model.2015
Author(s)
Arima H, Omura T, Hayasaka T, Masaki N, Hanada M, Xu D, Banno T, Kobayashi K, Takeuchi H, Kadomatsu K, Matsuyama Y, Setou M.
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Journal Title
Neuroscience
Volume: 297
Pages: 127-136
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Blockade of IL-6 signaling by MR16-1 inhibits reduction of docosahexaenoic acid-containing phosphatidylcholine levels in a mouse model of spinal cord injury.2014
Author(s)
Arima H, Hanada M, Hayasaka T, Masaki N, Omura T, Xu D, Hasegawa T, Togawa D, Yamato Y, Kobayashi S, Yasuda T, Matsuyama Y, Setou M.
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Journal Title
Neuroscience
Volume: 269
Pages: 1-10
Related Report
Peer Reviewed
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[Presentation] MR16-1 allows recovery from spinal cord injury: An evaluation-based study using imaging mass spectrometry.2013
Author(s)
Hideyuki Arima, Mitsuru Hanada, Takahiro Hayasaka, Noritaka Masaki, Tomohiko Hasegawa, Daisuke Togawa, Yu Yamato, Sho Kobayashi, Mitsutoshi Setou, Yukihiro Matsuyama
Organizer
8th tri-annual Combined Meeting of Orthopaedic Research Societies
Place of Presentation
Venice, Italy
Related Report
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