| Project/Area Number |
24592194
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Shiga University of Medical Science |
|---|
Principal Investigator |
MORI Kanji 滋賀医科大学, 医学部, 講師 (30467386)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
IMAI Shinji 滋賀医科大学, 医学部, 准教授 (90283556)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Keywords | BDNF / 末梢神経障害 / 神経修復 |
|---|
| Outline of Final Research Achievements |
Brain-derived neurotrophic factor (BDNF) stimulates peripheral nerve regeneration. However, the origin of BNDF and its precise effect on nerve repair have not been clarified. In the present study, we examined the role of BDNF from bone marrow derived cells (BMDCs) in post-injury nerve repair. For the evaluation of BDNF from BMDCs, studies with bone marrow transplantation (BMT) were performed.
We found that nerve function of BDNF knockout mice was significantly lower than that of control mice. BDNF expression was very faint before injury, but was dramatically increased after injury in the distal segment from the crush site. BDNF expression was mainly co-localized with CD45 in BMDCs, which was further confirmed by the appearance of GFP-positive cells in the BMT study. These results suggested that BDNF played an important role in peripheral nerve repair. In addition, BMDCs were recruited for the nerve injury site and BDNF derived from these BMDCs has an essential role in nerve repair.
|
