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The effect of autophagy activator to neural cells under hypoxic condition

Research Project

Project/Area Number 24592204
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionKagoshima University

Principal Investigator

TANABE Fumito  鹿児島大学, 医歯(薬)学総合研究科, 助教 (90619199)

Co-Investigator(Kenkyū-buntansha) KOMIYA Setsuro  鹿児島大学, 大学院医歯学総合研究科, 教授 (30178371)
SETOGUCHI Takao  鹿児島大学, 大学院医歯学総合研究科, 特任准教授 (40423727)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords神経幹細胞 / iPS細胞 / オートファジー / 低酸素 / 脊髄損傷 / 慢性圧迫性脊髄障害
Outline of Final Research Achievements

We used the neural stem cell cell line AF22 which is derived by human iPS cell.The expression of LC3-II, which is a marker of autophagy, was upregulated in 1%O2 condition compared to 20% O2condition. LiCl which is an autophagy activator increased the proliferation of AF22. In addition, rapamycin which is an another autophagy activator increased the proliferation of AF22 under 1% O2 and 20% O2 conditions. Furthermore, differentiation capacity of AF22 was not chaged under 1% O2 condition.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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