Gene transfection with endogenous opioid in the intractable pain models and cancer pain models.

• Project/Area Number: 24592211
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Teikyo University
• Principal Investigator: OZAWA TOMOYUKI 帝京大学, 医学部, 講師 (60375706)
• Co-Investigator(Kenkyū-buntansha): TOYONE Tomoaki 帝京大学, 医学部, 教授 (10407918) ; OOTORI Seiji 千葉大学, 医学部付属病院, 助教 (40361430)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 難治性疼痛 / 内因性オピオイド / 体外衝撃波 / 遺伝子導入 / 神経障害性疼痛 / 椎間板性腰痛 / proopiomelanocortin / 炎症性疼痛 / がん性疼痛 / オピオイド受容体 / 国際情報交換

Outline of Final Research Achievements

We established the new cancer pain model in rats to investigate new treatment for the cancer pain. Furthermore, this study evaluated the effects of POMC (proopiomelanocortin, the precursor for β-endorphin, an endogenous opioid) gene transfection using radial shock waves (RSWs). We used mainly two models; neuropathic pain model, cancer pain model. POMC gene transfection suppressed CGRP expression in the DRGs, microglial activation in the dorsal horn of the spinal cord, and pain behavior These findings suggest the effectiveness of POMC gene transfection using RSWs for the treatment of neuropathic pain, and cancer pain, without side effects for a long time.

Research Products

• [Journal Article] In vivo transfection of proopiomelanocortin gene, precursor of endogenous endorphin, by radial shock waves alleviates neuropathic pain (2013)
  • Author(s): TETSUHIRO ISHIKAWA,MASAYUKI MIYAGI,MASAOMI YAMASHITA,HIROTO KAMODA,YAWARA EGUCHI,GEN ARAI,MIYAKO SAZUKI,YOSHIHIRO SAKUMA,YASUHIRO OIKAWA,SUMIHISA ORITA,GEN INOUE,TOMOYUKI OZAWA,YASUCHIKA AOKI,TOMOAKI TOYONE,KAZUHISA TAKAHASHI,ATSUSHI YAMAGUCHI,ＳＥＩＪＩ ＯＨＴＯＲＩ
  • Journal Title: Journal of Orthopedic Science Volume: Jul; 18(4) Issue: 4 Pages: 636-45
  • DOI: 10.1007/s00776-013-0397-y
  • Peer Reviewed