| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Introduction; We focused on CD146, a marker for mesenchymal stem cells and detected in the cultured mouse annulus fibrosus (AF) cells to know whether it is a progenitor marker to restore functional AF tissue. AF cell culture was subjected to mesenchymal differentiation assay. Effects of transforming growth factor β-1 on CD146 expression was tested. Sorted CD146+ cells and CD146- cells were compared in their mRNA and protein expression. Morphology and contractile ability in three dimensional collagen gels were also determined.
Results: CD146+ cells showed only weak osteogeneic potential. Expression of CD146 was up regulated by TGFβ-1. In CD146+ cells, significantly higher expression of mRNA of smooth muscle protein 22 alpha and higher collagen gel contractile ability were detected. Our findings suggest that CD146+ cells are differentiated AF cells that are highly contractile and capable of interaction between cell-cell or cell-matrix for remodeling functional AF tissue-like structure.
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