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Isolation and application of progenitor cells from annulus fibrosus of the intervertebral disc

Research Project

Project/Area Number 24592212
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionTokai University

Principal Investigator

NAKAI Tomoko  東海大学, 医学部, 特定研究員 (20624589)

Co-Investigator(Renkei-kenkyūsha) SAKAI Daisuke  東海大学, 医学部, 准教授 (10408007)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords椎間板再生医療 / 線維輪組織再構築 / 線維輪培養細胞 / 前駆細胞 / 細胞表面マーカー / コラーゲンゲル収縮 / 椎間板線維輪 / 線維輪細胞 / 表面マーカー / ソーティング / CD146 / 細胞骨格 / コラーゲン繊維 / 配向 / フレキシブル / 国際研究者交流、香港 / 国際情報交換、スイス / 国際情報交換、ドイツ
Outline of Final Research Achievements

Introduction; We focused on CD146, a marker for mesenchymal stem cells and detected in the cultured mouse annulus fibrosus (AF) cells to know whether it is a progenitor marker to restore functional AF tissue. AF cell culture was subjected to mesenchymal differentiation assay. Effects of transforming growth factor β-1 on CD146 expression was tested. Sorted CD146+ cells and CD146- cells were compared in their mRNA and protein expression. Morphology and contractile ability in three dimensional collagen gels were also determined.
Results: CD146+ cells showed only weak osteogeneic potential. Expression of CD146 was up regulated by TGFβ-1. In CD146+ cells, significantly higher expression of mRNA of smooth muscle protein 22 alpha and higher collagen gel contractile ability were detected. Our findings suggest that CD146+ cells are differentiated AF cells that are highly contractile and capable of interaction between cell-cell or cell-matrix for remodeling functional AF tissue-like structure.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (4 results)

All 2014 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results)

  • [Journal Article] Effect of cryopreservation on canine and human activated nucleus pulposus cells: a feasibility study for cell therapy of the intervertebral disc.2013

    • Author(s)
      田中真弘、酒井大輔、檜山明彦、新井文征、中島大輔、額賀唯至、中井知子、持田譲治
    • Journal Title

      BioResearch

      Volume: 2 Issue: 4 Pages: 273-282

    • DOI

      10.1089/biores.2013.0023

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc.2012

    • Author(s)
      Sakai, D. et al.
    • Journal Title

      Nature Comm.

      Volume: 3 Issue: 1 Pages: 1264-1264

    • DOI

      10.1038/ncomms2226

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] マウス線維輪培養細胞に発現するCD146の機能2014

    • Author(s)
      中井知子、中村嘉彦、酒井大輔、持田譲治
    • Organizer
      第29回日本整形外科学会基礎学術集会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2014-10-09 – 2014-10-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] マウス線維輪培養細胞の分化過程におけるCD146の発現について2013

    • Author(s)
      中井知子、中村嘉彦、酒井大輔、持田譲治
    • Organizer
      第28回日本整形外科学会基礎学術集会
    • Place of Presentation
      幕張メッセ国際会議場(千葉市)
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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