Isolation and application of progenitor cells from annulus fibrosus of the intervertebral disc
| Project/Area Number |
24592212
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokai University |
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Principal Investigator |
NAKAI Tomoko 東海大学, 医学部, 特定研究員 (20624589)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKAI Daisuke 東海大学, 医学部, 准教授 (10408007)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 椎間板再生医療 / 線維輪組織再構築 / 線維輪培養細胞 / 前駆細胞 / 細胞表面マーカー / ｺﾗｰｹﾞﾝｹﾞﾙ収縮 / 椎間板線維輪 / 線維輪細胞 / 表面マーカー / ソーティング / CD146 / 細胞骨格 / ｺﾗｰｹﾞﾝ繊維 / 配向 / フレキシブル / 国際研究者交流、香港 / 国際情報交換、スイス / 国際情報交換、ドイツ |
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| Outline of Final Research Achievements |
Introduction; We focused on CD146, a marker for mesenchymal stem cells and detected in the cultured mouse annulus fibrosus (AF) cells to know whether it is a progenitor marker to restore functional AF tissue. AF cell culture was subjected to mesenchymal differentiation assay. Effects of transforming growth factor β-1 on CD146 expression was tested. Sorted CD146+ cells and CD146- cells were compared in their mRNA and protein expression. Morphology and contractile ability in three dimensional collagen gels were also determined.
Results: CD146+ cells showed only weak osteogeneic potential. Expression of CD146 was up regulated by TGFβ-1. In CD146+ cells, significantly higher expression of mRNA of smooth muscle protein 22 alpha and higher collagen gel contractile ability were detected. Our findings suggest that CD146+ cells are differentiated AF cells that are highly contractile and capable of interaction between cell-cell or cell-matrix for remodeling functional AF tissue-like structure.
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Report
(4 results)
Research Products
(4 results)
