Analysis of appropriate cell source for efficient annulus fibrosis regeneration
| Project/Area Number |
24592213
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MOCHIDA Joji 東海大学, 医学部, 教授 (30129697)
HIYAMA Akihiko 東海大学, 医学部, 講師 (00514382)
NAKAI Tomoko 東海大学, 医学部, 特定研究員 (20624589)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 椎間板 / 再生医療 / 幹細胞 / 線維輪 / 再生医学 / 線維輪細胞 |
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| Outline of Final Research Achievements |
Altered function of the intervertebral disc leads to various degenerative spine disorders. We established an isolation method for retrieving purely annulus fibrosus cells from intervertebral disc of C57BL/6 mouse. We then tested whether CD146, a marker for mesenchymal stem cells in various organs, relates to proliferation and differentiation of annulus fibrosis cell. We isolated AF tissue from mouse and cultured isolated cells to analyze their cell surface markers and multi-potency. Result showed that mouse annulus fibrosus cells differentiated to three mesenchymal lineages. Furthermore, CD 146 positive annulus fibrosus cells became proliferative with hypoxia, TGF-beta 1 or R3-IGF1 stimuli suggesting its role as a useful differentiation marker for annulus fibrosus regeneration.
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Report
(4 results)
Research Products
(14 results)
