Bone regeneration using culture-expanded bone marrow cells-Efficacy of Runx2-activating drugs-
Project/Area Number |
24592230
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Orthopaedic surgery
|
Research Institution | Nagoya University |
Principal Investigator |
HIROSHI Kitoh 名古屋大学, 医学(系)研究科(研究院), 准教授 (40291174)
|
Co-Investigator(Kenkyū-buntansha) |
金子 浩史 名古屋大学, 医学(系)研究科(研究院), 助教 (60566975)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | Runx2 / ランソプラゾール / 骨組織再生 / 培養骨髄細胞 / 骨芽細胞 / 骨形成 / 骨形成促進 / drug repositioning |
Outline of Final Research Achievements |
We screened for drugs that activate the Runx2 promoter and identified that lansoprazole, a proton pump inhibitor, upregulated the Runx2 activity. Lansoprazole elevated expression levels of Runx2 mRNA and protein in osteoblast-lineage cells in a dose-dependent manner. Lansoprazole exerted an increase of ALP and Osterix activities in osteoblast-lineage cells. Lansoprazole accelerated the matrix calcium deposition in human bone marrow derived osteoprogenitors. Systemic administration of lansoprazole to a rat fracture model increased osteoblastic parameters and facilitated fracture healing. We propose that lansoprazole holds promise as a therapeutic agent for bone regeneration at fracture sites.
|
Report
(4 results)
Research Products
(38 results)
-
-
-
-
-
-
-
[Journal Article] Mutations in PCYT1A, encoding a key regulator of phosphatidylcholine metabolism, cause spondylometaphyseal dysplasia with cone-rod dystrophy.2014
Author(s)
Hoover-Fong J, Sobreira N, Jurgens J, Modaff P, Blout C, Moser A, Kim OH, Cho TJ, Cho SY, Kim SJ, Jin DK, Kitoh H, Park WY, Ling H, Hetrick KN, Doheny KF, Valle D, Pauli RM.
-
Journal Title
Am J Hum Genet
Volume: 94(1)
Issue: 1
Pages: 105-112
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
[Journal Article] Meclozine facilitates proliferation and differentiation of chondrocytes by attenuating abnormally activated FGFR3 signaling in achondroplasia.2013
Author(s)
Matsushita, M., Kitoh, H., Ohkawara, B., Mishima, K., Kaneko, H., Ito, M., Masuda, A., Ishiguro, N. and Ohno, K.
-
Journal Title
PLOS ONE
Volume: 8(12)
Issue: 12
Pages: 81569-81569
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-