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The pretreatment of nerve autograft used in the reimplantation surgery after nerve root avulsion injury

Research Project

Project/Area Number 24592232
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionKyoto University

Principal Investigator

SOUICHI Ohta  京都大学, 医学(系)研究科(研究院), 助教 (70592484)

Co-Investigator(Kenkyū-buntansha) KAKINOKI Ryosuke  近畿大学, 医学部, 教授 (20314198)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords神経根引き抜き損傷 / 治療 / ラット / 神経移植 / Resveratrol / 腕神経叢損傷 / 脊髄損傷 / 神経再生 / 運動神経
Outline of Final Research Achievements

We investigated the pretreatment of the nerve autograft used in rat reimplantation surgery of the avulsed cervical nerve root. The nerve autograft was harvested from 5th cervical nerve root when 6th cervical nerve root was extravertebrally avulsed, and cultured in the commercially available Schwann cell medium for one week prior to the reimplantation surgery. Then, the cultured nerve was grafted to bridge between the avulsed spinal cord segment and the distal end of the avulsed nerve root one week after the first surgery. The histological examination at 8 weeks after second surgery showed that the addition of 1nM erythropoietin to the culture medium had no effects on axonal regeneration and survival of motor neurons in the lesioned side of the spinal cord segment. The addition of 100µM resveratrol to the culture medium showed marked increase of axonal regeneration in the 2mm distal to the distal coaptation site of the grafted nerve, although sample size was small.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2015 2014 2013 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results)

  • [Journal Article] ラット頚髄神経の節前損傷と節後損傷における組織学的所見の比較検討2014

    • Author(s)
      野口貴志、太田壮一、貝澤幸俊、織田宏基、柿木良介、松田秀一
    • Journal Title

      日本手外科学会誌

      Volume: 31 Pages: 488-491

    • NAID

      40020389580

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new cervical nerve root avulsion model using a posterior extra-vertebral approach in rats.2013

    • Author(s)
      Noguchi T, Ohta S, Kakinoki R, Kaizawa Y, Matsuda S.
    • Journal Title

      J Brachial Plex Peripher Nerve Inj.

      Volume: 8 Issue: 01 Pages: 8-16

    • DOI

      10.1186/1749-7221-8-8

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Histological Comparison Between Pre- And Postganglionic Injuries of The Brachial Plexus in a rat model2015

    • Author(s)
      Takashi Noguchi, Souichi Ohta, Yukitoshi Kaizawa, Ryosuke, Ikeguchi, Hiroki Oda, Ryosuke Kakinoki, Shuichi Matsuda
    • Organizer
      61th Annual Meeting of the Orthopaedic Research Society
    • Place of Presentation
      Las Vegas(USA)
    • Year and Date
      2015-03-28 – 2015-03-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] ラット頚髄神経の節前損傷と節後損傷における組織学的所見の比較検討2014

    • Author(s)
      野口貴志、太田壮一、柿木良介、貝澤幸俊、松田秀一
    • Organizer
      第57回日本手外科学会学術集会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2014-04-17 – 2014-04-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Histological Comparison Between the Preganglionic and Postganglionic Injuries of Brachial Plexus in a Rat Model2013

    • Author(s)
      Takashi Noguchi, Souichi Ohta, Yukitoshi Kaizawa, Ryosuke Kakinoki, Shuichi Matsuda
    • Organizer
      68th annual meeting of the American Society for Surgery of the Hand
    • Place of Presentation
      Moscone West Convention Center(San Francisco, CA, USA)
    • Related Report
      2013 Research-status Report
  • [Presentation] ラット神経根引き抜き損傷におけるerythropoietinの投与開始時期と運動神経細胞死抑制効果についての検討

    • Author(s)
      野口貴志、太田壮一、柿木良介、貝澤幸俊、松田秀一
    • Organizer
      第28回日本整形外科学会基礎学術集会
    • Place of Presentation
      幕張メッセ(千葉市)
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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