Therapeutic strategy based on angiogenesis induce by microRNA in orthopaedic field
| Project/Area Number |
24592234
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OCHI Mitsuo 広島大学, 大学院医歯薬保健学研究院, 教授 (70177244)
MIYAKI Shigeru 広島大学, 病院, 講師 (10392490)
KAMEI Naosuke 広島大学, 病院, 病院助教 (70444685)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | microRNA / 血管新生 / エクソソーム / 血管再生 / ｴｸｿｿｰﾑ |
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| Outline of Final Research Achievements |
The therapeutic effect of microRNA (miRNA) which play a role in angiogenesis on injury in orthpaedic field. Especially, we focused on secreted miRNAs from cell. Peripheral blood mononucleated cells were isolated from healthy subjects, and cultured. Stimuli by hypoxia environment was given during culture, and the medium with or without hypoxia were analyzed using miRNA microarray. Several miRNAs showed specific expression pattern, and over expression of these miRNAs in fibroblast induced cell proliferation. In vivo, administration of miRNA related to angiogenesis into rat model of meniscus, tendon injury and atrophic non-union showed successful results.
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Report
(4 results)
Research Products
(7 results)
