Exosome-formed miRNA for the treatment of osteosarcoma

Research Project

Project/Area Number	24592235
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Orthopaedic surgery
Research Institution	Hiroshima University
Principal Investigator	SHIMOSE Shoji 広島大学, 大学院医歯薬保健学研究院(医), 准教授 (30304439)
Co-Investigator(Kenkyū-buntansha)	OCHI Mitsuo 広島大学, 大学院医歯薬保健学研究院, 教授 (70177244) KUBO Tadahiko 広島大学, 病院, 講師 (70397959) MIYAKI Shigeru 広島大学, 病院, 講師 (10392490)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	骨肉腫 / microRNA / エクソソーム / 転移
Outline of Final Research Achievements	MicroRNAs (miRNAs) have emerged as potential anticancer agents, but their clinical application is limited by the lack of an effective delivery system to tumors. Exosomes are small vesicles that play important roles in intercellular communication. Here, we show that synthetic miR-143 introduced into cells is released enveloped in exosomes and that the secreted exosome-formed miR-143 is transferred to osteosarcoma cells. The delivery of exosome-formed miR-143 significantly reduced the migration of osteosarcoma cells. The delivery efficiency of exosome-formed miR-143 was less than that achieved with lipofection, but the migratory potential of osteosarcoma cells was similarly inhibited after both strate- gies. Our results suggest that exosomes can deliver synthetic miR-143 and are a potentially efficient and functional delivery system.