GLI2-targeted gene therapy for musculoskeletal tumors
Project/Area Number |
24592237
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Orthopaedic surgery
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Research Institution | Kagoshima University |
Principal Investigator |
NAGANO Satoshi 鹿児島大学, 医歯(薬)学総合研究科, 講師 (50373139)
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Co-Investigator(Kenkyū-buntansha) |
KOMIYA Setsuro 鹿児島大学, 医歯学総合研究科, 教授 (30178371)
SETOGUCHI Takao 鹿児島大学, 医歯学総合研究科, 特任准教授 (40423727)
KOSAI Kenichiro 鹿児島大学, 医歯学総合研究科, 教授 (90258418)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | 遺伝子治療 / ヘッジホッグシグナル / 骨軟部腫瘍 / GLI2 / Adenovirus / Hedgehog / RPS3 / ヘッジホッグ経路 / サバイビン / がん幹細胞 / 骨肉腫 |
Outline of Final Research Achievements |
We showed arsenic trioxide targets GLI2, a crucial factor for intracellular signaling of musculoskeletal tumors, and suppressed growth of osteosarcoma cells. ATO is already approved for leukemia, therefore translation into clinical usage for osteosarcoma is anticipated. We also showed that Surv.m-CRA, a survivin-responsive conditionally-replicative adenovirus (CRA), exhibit strong antitumor activity to rhabdomyosarcoma stem cells. We found that ribosomal protein S3 is a target gene of GLI2 and its knockdown suppressed invasion and motility of osteosarcoma cells. We are preparing for clinical trial of Surv.m-CRA for musculoskeletal tumor patients, which is expected to further advance the research of gene therapy for musculoskeletal tumors.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] GLI2 is a novel therapeutic target for metastasis of osteosarcoma.2015
Author(s)
Nagao-Kitamoto H, Nagata M, Nagano S, Kitamoto S, Ishidou Y, Yamamoto T, Nakamura S, Tsuru A, Abematsu M, Fujimoto Y, Yokouchi M, Kitajima S, Yoshioka T, Maeda S, Yonezawa S, Komiya S, Setoguchi T.
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Journal Title
Int J Cancer.
Volume: 136
Issue: 6
Pages: 1276-1284
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Ribosomal protein S3 regulates GLI2-mediated osteosarcoma invasion.2015
Author(s)
Nagao-Kitamoto H, Setoguchi T, Kitamoto S, Nakamura S, Tsuru A, Nagata M, Nagano S, Ishidou Y, Yokouchi M, Kitajima S, Yoshioka T, Maeda S, Yonezawa S, Komiya S.
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Journal Title
Cancer Lett.
Volume: 356
Issue: 2
Pages: 855-861
DOI
Related Report
Peer Reviewed
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[Journal Article] Human Immunodeficiency Virus Type I Enhancer Binding Protein 3 is Essential for the Expression of Asparagine-linked Glycosylation 2 in the Regulation of Osteoblast and Chondrocyte Differentiation.2014
Author(s)
Imamura K, Maeda S, Kawamura I, Matsuyama K, Shinohara N, Yahiro Y, Nagano S, Setoguchi T, Yokouchi M, Ishidou Y, Komiya S.
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Journal Title
Journal of Biological Chemistry
Volume: 289
Issue: 14
Pages: 9865-9879
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] RBPJ Is a Novel Target for Rhabdomyosarcoma Therapy.2012
Author(s)
Hiroko Nagao, Takao Setoguchi, Sho Kitamoto, Yasuhiro Ishidou, Satoshi Nagano, Masahiro Yokouchi, Masahiko Abematsu, Naoya Kawabata, Shingo Maeda, Suguru Yonezawa, Setsuro Komiya
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Journal Title
PLoS ONE
Volume: 7(7)
Issue: 7
Pages: e39268-e39268
DOI
Related Report
Peer Reviewed
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[Journal Article] Pathological femoral fractures due to osteomalacia associated with adefovir dipivoxil treatment for hepatitis B: a case report.2012
Author(s)
Tanaka M, Setoguchi T, Ishidou Y, Arishima Y, Hirotsu M, Saitoh Y, Nakamura S, Kakoi H, Nagano S, Yokouchi M, Kamizono J, Komiya S.
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Journal Title
Diagn Pathol
Volume: 7
Issue: 1
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] Arsenic trioxide prevents osteosarcoma growth by inhibition of GLI transcription via DNA damage accumulation2013
Author(s)
S Nakamura, S Nagano, H Nagao, A Tsuru , Y Ishidou, M Yokouchi, M Abematsu, T Yamamoto, S Komiya, T Setoguchi
Organizer
Orthopedic Research Society 2013 Annual Meeting
Place of Presentation
San Antonio, TX, USA
Related Report
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