• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Inhibition of the Hedgehog pathway prevents human rhabdomyosarcoma cell growth

Research Project

Project/Area Number 24592238
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionKagoshima University

Principal Investigator

KAWABATA Naoya  鹿児島大学, 医歯(薬)学総合研究科, 客員研究員 (60626836)

Co-Investigator(Kenkyū-buntansha) KOMIYA Setsuro  鹿児島大学, 大学院医歯学総合研究科, 教授 (30178371)
SETOGUCHI Takao  鹿児島大学, 大学院医歯学総合研究科, 特任准教授 (40423727)
NAGANO Satoshi  鹿児島大学医学部, 歯学部附属病院, 講師 (50373139)
ABEMATSU Masahiko  鹿児島大学医学部, 歯学部附属病院, 助教 (70448190)
TANABE Fumito  鹿児島大学医学部, 歯学部附属病院, 助教 (90619199)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords横紋筋肉腫 / Hedgehog / GLI2 / 骨肉腫 / 骨軟部肉腫 / 転移 / Notch / RBPJ
Outline of Final Research Achievements

We investigated the expression and function of the Hedgehog pathway. Real-time PCR revealed that human rhabdomyosarcoma cell lines and biopsy specimens overexpressed the following genes: Sonic hedgehog, Indian hedgehog, Desert hedgehog, PTCH1, SMO, GLI1, GLI2 and ULK3. Immunohistochemistry revealed that rhabdomyosarcoma cell lines and biopsy specimens expressed SMO and GLI2. Inhibition of SMO by cyclopamine slowed the growth of human rhabdomyosarcoma cell lines. Similarly, inhibition of GLI by GANT61 slowed the growth of human rhabdomyosarcoma cell lines. Inhibition of cell proliferation and apoptotic cell death together prevented the growth of rhabdomyosarcoma cells by cyclopamine and GANT61 treatment. In addition, knockdown of GLI2 inhibited invasion of osteosarcoma cell and decreased lung metastasis of osteosarcomas. Our findings suggest that inhibition of the Hedgehog pathway may be a useful approach for treating rhabdomyosarcoma and osteosarcoma patients.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (4 results)

All 2015 2014 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results)

  • [Journal Article] GLI2 is a novel therapeutic target for metastasis of osteosarcoma.2015

    • Author(s)
      Nagao-Kitamoto H, Nagata M, Nagano S, Kitamoto S, Ishidou Y, Yamamoto T, Nakamura S, Tsuru A, Abematsu M, Fujimoto Y, Yokouchi M, Kitajima S, Yoshioka T, Maeda S, Yonezawa S, Komiya S, Setoguchi T.
    • Journal Title

      Int J Cancer.

      Volume: 136 Issue: 6 Pages: 1276-1284

    • DOI

      10.1002/ijc.29107

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Survivin-responsive conditionally replicating adenovirus kills rhabdomyosarcoma stem cells more efficiently than their progeny2014

    • Author(s)
      Tanoue K, Wang Y, Ikeda M , Mitsui K, Irie R , Setoguchi T, Komiya S, Natsugoe S, Kosai K
    • Journal Title

      J Trans Med

      Volume: 12 Issue: 1 Pages: 27-27

    • DOI

      10.1186/1479-5876-12-27

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Arsenic trioxide prevents osteosarcoma growth by inhibition of GLI transcription via DNA damage accumulation.2013

    • Author(s)
      Nakamura S, Nagano S, Nagao H, Ishidou Y, Yokouchi M, Abematsu M, Yamamoto T, Komiya S, Setoguchi T.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 7 Pages: e69466-e69466

    • DOI

      10.1371/journal.pone.0069466

    • NAID

      120005324788

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] RBPJ Is a Novel Target for Rhabdomyosarcoma Therapy.2012

    • Author(s)
      Hiroko Nagao, Takao Setoguchi, Sho Kitamoto, Yasuhiro Ishidou, Satoshi Nagano, Masahiro Yokouchi, Masahiko Abematsu, Naoya Kawabata, Shingo Maeda, Suguru Yonezawa, Setsuro Komiya
    • Journal Title

      PLoS ONE

      Volume: 7(7) Issue: 7 Pages: e39268-e39268

    • DOI

      10.1371/journal.pone.0039268

    • Related Report
      2012 Research-status Report
    • Peer Reviewed

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi