| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Outline of Final Research Achievements |
As a surgical method of reconstruction following resecting large musculoskeletal tumors while considering the preservation of limbs, autogenous bone treated is selected. However, not only tumor cells but intact cells are killed in the treated bone. Therefore, the treated bone loses osteogenic ability. The aim of this study was to develop treated bone with osteogenic capability. We gained autologous bone marrow-derived mesenchymal cells (BMSCs) by using tissue-engineered technique and transferred these BMSCs to autologous bone treated with liquid nitrogen in an animal experimental model. The BMSCs-seeded treated bones were ectopically transplanted into subcutaneous rat sites. After that, their osteogenic capabilities were evaluated by biochemical and histological evaluations. These results indicated the suitability of tissue-engineered BMSCs to heighten osteogenic response. The autograft bone treated with liquid nitrogen was given osteogenic capability by using tissue-engineered BMSCs.
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