| Project/Area Number |
24592247
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKASHO Keiji 兵庫医科大学, 医学部, 教授 (00217712)
YAMANEGI Koji 兵庫医科大学, 医学部, 講師 (00434944)
YAMADA Naoko 兵庫医科大学, 医学部, 講師 (10319858)
HATA Masaki 兵庫医科大学, 医学部, 研究生(研究員) (10446057)
OHYAMA Hideki 兵庫医科大学, 医学部, 非常勤講師 (90280685)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 骨肉腫 / 薬剤耐性 / 非ステロイド系抗炎症剤 |
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| Outline of Final Research Achievements |
We investigated that the combination of histone deacetylase (HDAC) inhibitor, hydralazine (DNA methylation inhibitor) and celecoxib (nonsteroidal anti-inflammatory drugs, cyclooxygenase-2 inhibitor) inhibit inducing the expression of multidrug resistance (MDR) protein (MDR-1, MRP-1) in osteosarcoma cells without inhibiting cytotoxic sensitivity. The combination of HDAC inhibitor and celecoxib significantly inhibits cell-proliferation. This combination showed different reactions depending on the type of cells but generally inhibited. HDAC inhibitor alone increased MDR activity, however, combined with celecoxib reduced the activity depended on MDR-1 and MRP-1. These results suggest that combined treatment of HDAC inhibitor, hydralazine and celecoxib may be a useful for enhancing the effect of chemotherapy for osteosarcoma.
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