mmunomodulation of recipient mesenchymal stem cells in composite tissue allotransplantation

• Project/Area Number: 24592249
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kyoto University (2014); Foundation for Biomedical Research and Innovation (2012-2013)
• Principal Investigator: IKEGUCHI Ryosuke 京都大学, 医学(系)研究科(研究院), 准教授 (80437201)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 間葉系幹細胞 / 同種移植 / 移植 / 四肢移植 / 複合組織移植 / 複合組織同種移植 / 拒絶反応 / 免疫抑制 / 再生 / 免疫

Outline of Final Research Achievements

Hand transplantations have revolutionized the reconstruction field for patients with hand defects. However, immunosuppressants are essential for maintaining human hand transplant survival despite lethal side effects. Mesenchymal stem cells (MSCs) have some immunomodulatory properties to suppress T cell mediate responses. The purpose of this study is to evaluate the effect of intravenous recipient MSCs infusion for immunomodulation in the rat composite tissue allotransplantation model. The graft survival of MSCs infusion group was significantly prolonged in comparison with that of control group. Cytokine expression analysis showed that MSCs treatment significantly decreased pro-inflammatory cytokine expression. An in vitro mixed lymphocyte reaction showed MSCs inhibiting T cell proliferation.
MSCs demonstrate some immuno-modulatory properties that can be accomplished without the need for significant recipient immunosuppression.

Research Products

• [Presentation] Immunomodulation of recipient mesenchymal stem cells in composite tissue allotransplantation (2014)
  • Author(s): Ryosuke Ikeguchi, Ryosuke Kakinoki, Hiroto Mitsui, Tomoki Aoyama, Tadashi Yasuda, Junya Toguchida, Shuichi Matsuda
  • Organizer: American Academy of Orthopaedic Surgeons
  • Place of Presentation: アメリカ合衆国