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Alterations in the Chondroitin Sulfate Chain in Human Osteoarthritic Cartilage of the Knee

Research Project

Project/Area Number 24592258
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionGifu University

Principal Investigator

MATSUMOTO Kazu  岐阜大学, 医学部附属病院, 准教授 (40422711)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsコンドロイチン硫酸 / 変形性膝関節症
Outline of Final Research Achievements

The aim of this study is to determine whether the structure of chondroitin sulfate (CS) in cartilage is reflected by the degree of cartilage degeneration in patients with osteoarthritis (OA) of the knee. Two osteoarthritic cartilage samples were obtained from each femoral condyle of 24 knees with end-stage OA. Histological grade was determined according to the Mankin score. The CS concentrations and chain length were determined using HPLC and gel filtration chromatography, respectively. Expression of the gene encoding CS glycosyltransferase was evaluated using qPCR. As a results, the mean CS concentration and chain length were significantly lower and shorter in the more degraded cartilage. Genes expressions encoding CS glycosyltransferases were expressed at lower levels in the degraded cartilage. In the osteoarthritic knee, the CS concentration and chain length were reduced closer to the more degraded cartilage with decreasing CS glycosyltransferase gene expression.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (3 results)

All 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Alterations in the Chondroitin Sulfate Chain in Human Osteoarthritic Cartilage of the Knee2014

    • Author(s)
      Ishimaru D, Sugiura N, Akiyama H, Watanabe H, Matsumoto K
    • Journal Title

      Osteoarthritis and cartilage

      Volume: 22(2) Issue: 2 Pages: 250-258

    • DOI

      10.1016/j.joca.2013.11.010

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 末期変形性膝関節症における関節軟骨内コンドロイチン硫酸鎖の構造変化2014

    • Author(s)
      石丸 大地、杉浦 信夫、秋山 治彦、渡辺 秀人、松本 和
    • Organizer
      第29回日本整形外科基礎学術集会
    • Place of Presentation
      城山観光ホテル 鹿児島市
    • Year and Date
      2014-10-09 – 2014-10-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 末期変形性膝関節症における関節軟骨内コンドロイチン硫酸鎖の構造変化2014

    • Author(s)
      石丸 大地、杉浦 信夫、秋山 治彦、渡辺 秀人、松本 和
    • Organizer
      第32回日本骨代謝学会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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