| Project/Area Number |
24592260
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka University |
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Principal Investigator |
KUNUGIZA Yasuo 大阪大学, 医学(系)研究科(研究院), 招へい教員 (60507193)
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| Co-Investigator(Kenkyū-buntansha) |
TOMITA Tetsuya 大阪大学, 大学院医学系研究科, 准教授 (30283766)
YOSHIKAWA Hideki 大阪大学, 大学院医学系研究科, 教授 (60191558)
TANIYAMA Yoshiaki 大阪大学, 大学院医学系研究科・寄附講座, 准教授 (60372611)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ペリオスチン / 変形性関節症 / 軟骨 / 炎症 / ＭＭＰ / ぺリオスチン / 軟骨細胞 / MMPs / IL-6 / ノックアウトマウス |
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| Outline of Final Research Achievements |
We performed the study to examine the expression and function of periostin in human OA. The human cartilage samples were obtained from the knees of patients undergoing total knee arthroplasty as OA samples and from the femoral bone head of patients with femoral neck fracture as control samples. The periostin mRNA was significantly higher in OA cartilage than in control cartilage. Immunohistochemical analysis of periostin showed that the main positive signal was localized in chondrocytes and their periphery matrix near the erosive area. The expression of periostin was also detected in the fibrotic cartilage and fibrotic tissue of subchondral bone. In cultured human chondrocytes, periostin induced IL-6, 8, MMP-1, 3, 13, and NOS2 in a dose- and time-dependent manner. Inflammatory signaling by periostin was suppressed by NFκB inactivation in chondrocytes. Periostin was upregulated in OA cartilage, and it may amplify inflammatory events and accelerate OA pathology.
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