Search for novel osteoclast regulatory factors produced by bone marrow mesenchymal stem cells

• Project/Area Number: 24592263
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Okayama University
• Principal Investigator: FUJITA Hirofumi 岡山大学, 医歯(薬)学総合研究科, 助教 (20423288)
• Co-Investigator(Kenkyū-buntansha): OHMOTO Naoko 岡山大学, 大学院医歯薬学総合研究科, 非常勤研究員 (70598565) ; KOBUCHI Hirotsugu 岡山大学, 大学院医歯薬学総合研究科, 講師 (10304297)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 破骨細胞 / 骨髄間葉系幹細胞 / 炎症性サイトカイン / ハイスループットスクリーング / 質量分析 / 新規因子 / 破骨細胞形成

Outline of Final Research Achievements

We searched for the novel osteoclast regulatory factor produced by human bone marrow mesenchymal stem cell(hMSC) on inflammatory cytokine-induced osteoclast differentiation. Our findings showed that osteoclast differentiation by TNF-alpha was suppressed by conditioned medium of hMSC and we identified some candidate molecules of osteoclast regulatory factor using high-throughput screening and mass spectrometry.

Research Products

• [Journal Article] Erythroid and megakaryocytic differentiation of K562 erythroleukemic cells by monochloramine. (2014)
  • Author(s): Ogino T, Kobuchi H, Fujita H, Matsukawa A, Utsumi K.
  • Journal Title: Free Radic Res. Volume: 48 Issue: 3 Pages: 292-302
  • DOI: 10.3109/10715762.2013.865840
  • Peer Reviewed
• [Journal Article] Evolution of mammalian opn5 as a specialized UV-absorbing pigment by a single amino acid mutation (2014)
  • Author(s): Yamashita, T., Ono, K., Ohuchi, H., Yumoto, A., Gotoh, H., Tomonari, S., Sakai, K., Fujita, H., Imamoto, Y., Noji, S., Nakamura, K., and Shichida, Y.
  • Journal Title: J. Biol. Chem. Volume: 289 Issue: 7 Pages: 3991-4000
  • DOI: 10.1074/jbc.m113.514075
  • NAID: 120005690203
  • Peer Reviewed
• [Journal Article] Necrotic and apoptotic cells serve as nuclei for calcification on osteoblastic differentiation of human mesenchymal stem cells in vitro. (2014)
  • Author(s): Fujita H, Yamamoto M, Ogino T, Kobuchi H, Ohmoto N, Aoyama E, Oka T, Nakanishi T, Inoue K, Sasaki J
  • Journal Title: Cell Biochemistry & Function Volume: 32(1) Issue: 1 Pages: 77-86
  • DOI: 10.1002/cbf.2974
  • Peer Reviewed
• [Journal Article] The inhibition of ferrochelatase enhances 5-aminolevulinic acid-based photodynamic action for prostate cancer. (2013)
  • Author(s): Fukuhara H, Inoue K, Kurabayashi A, Furihata M, Fujita H, Utsumi K, Sasaki J, Shuin T
  • Journal Title: Photodiagnosis Photodyn Ther. Volume: 10 Issue: 4 Pages: 399-409
  • DOI: 10.1016/j.pdpdt.2013.03.003
  • Peer Reviewed
• [Journal Article] Improvement of the efficacy of 5-aminolevulinic acid-mediated photodynamic treatment in human oral squamous cell carcinoma HSC-4. (2013)
  • Author(s): Yamamoto M, Fujita H, Katase N, Inoue K, Nagatsuka H, Utsumi K, Sasaki J, Ohuchi H.
  • Journal Title: Acta Med Okayama. Volume: 67 Pages: 153-64
  • NAID: 120005295913
  • Peer Reviewed
• [Journal Article] Photodynamic therapy involves an antiangiogenic mechanism and is enhanced by ferrochelatase inhibitor in urothelial carcinoma. (2013)
  • Author(s): Inoue K, Fukuhara H, Kurabayashi A, Furihata M, Tsuda M, Nagakawa K, Fujita H, Utsumi K, Shuin T
  • Journal Title: Cancer Science Volume: 104(6) Issue: 6 Pages: 765-772
  • DOI: 10.1111/cas.12147
  • Peer Reviewed
• [Journal Article] Mitochondrial localization of ABC transporter ABCG2 and its function in 5-aminolevulinic acid-mediated protoporphyrin IX accumulation. (2012)
  • Author(s): Kobuchi H, Moriya K, Ogino T, Fujita H, Inoue K, Shuin T, Yasuda T, Utsumi K, Utsumi T
  • Journal Title: PLoS One Volume: 7 Issue: 11 Pages: e50082-e50082
  • DOI: 10.1371/journal.pone.0050082
  • Peer Reviewed
• [Journal Article] Resveratrol Inhibited Hydroquinone-induced Cytotoxicity in Mouse Primary Hepatocytes. (2012)
  • Author(s): (Wang DH), Otsuki Y, Fujita H, Miyazaki M, Yie Q, Tsutsui K, Sano K, Masuoka N, Ogino K.
  • Journal Title: Int J Environ Res & Public Health Volume: 9 Issue: 9 Pages: 3354-3364
  • DOI: 10.3390/ijerph9093354
  • Peer Reviewed
• [Presentation] Effect of glutathione on TNFα-induced osteoclast differentiation in murine bone marrow-derived macrophages (2015)
  • Author(s): 藤田 洋史、越智正彦、青山絵理子、荻野哲也、近藤洋一、大内淑代
  • Organizer: 第120回日本解剖学会総会・全国学術集会
  • Place of Presentation: 兵庫
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] TNFαによる破骨細胞分化に対する抗酸化物質グルタチオンの影響 (2014)
  • Author(s): 藤田 洋史、荻野哲也、青山絵理子、大内淑代
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] In vitroにおいてグルタチオンはTNFαによる破骨細胞分化を促進する (2014)
  • Author(s): 藤田 洋史、荻野哲也、青山絵理子、大内淑代
  • Organizer: 第67回日本酸化ストレス学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-09-04 – 2014-09-05
• [Presentation] TNFαによる破骨細胞形成とそれに対するα-トコフェロールの作用 (2014)
  • Author(s): 藤田 洋史
  • Organizer: 第17回Vitamin E update forum
  • Place of Presentation: 東京
  • Year and Date: 2014-08-06
  • Invited
• [Presentation] In vitroにおけるTNFαによる破骨細胞形成促進とグルタチオンの効果 (2014)
  • Author(s): 藤田 洋史、荻野哲也、青山絵理子、大内淑代
  • Organizer: 第32回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] TNFαによる破骨細胞形成と活性酸素生成に対する骨髄間葉系幹細胞の作用 (2014)
  • Author(s): 藤田 洋史、荻野哲也、佐々木順造、大内淑代
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 栃木
• [Presentation] Effects of bone marrow mesenchymal stem cells on TNF-alpha-induced osteoclast formation and ROS generation in bone marrow macrophages. (2014)
  • Author(s): Hirofumi Fujita, Tetsuya Ogino, Kobuchi Hirotsugu, Hideyo Ohuchi.
  • Organizer: 17th Biennial Meeting of Society for Free Radical Research International
  • Place of Presentation: 京都
• [Presentation] Oxidative stress and cell differentiation: monochloramine affects differentiation of K562 erythroleukemia cell line. (2014)
  • Author(s): Tetsuya Ogino, Hirotsugu Kobuchi, Hirofumi Fujita.
  • Organizer: 17th Biennial Meeting of Society for Free Radical Research International
  • Place of Presentation: 京都
• [Presentation] 骨芽細胞分化培養に伴う石灰化と活性酸素の関与 (2013)
  • Author(s): 藤田洋史、小渕 浩嗣、内海耕慥、佐々木順造、大内淑代
  • Organizer: 第30回日本骨代謝学
  • Place of Presentation: 横浜
• [Presentation] Sensitive detection and cell death induced by ALA-mediated photodynamic actions in adult T-cell leukemia/lymphoma cells (2013)
  • Author(s): 8. Takashi Oka, Hirofumi Fujita, Lamia Abd Al-Kader, Ichiro Murakami,Atae Utsunomiya and Tadashi Yoshino
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] TNFαによる破骨細胞形成に対する脂溶性抗酸化物質の効果 (2013)
  • Author(s): 藤田洋史、大本苗起子、荻野哲也、久木田敏夫、佐々木順造、大内淑代
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨
• [Presentation] 骨髄間葉系幹細胞のosteogenic cultureによる細胞死仲介性石灰化と活性酸素の関与 (2012)
  • Author(s): 藤田洋史、大本苗起子、永川恵介、小渕 浩嗣、荻野哲也、大内淑代、佐々木順造、内海耕慥
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] Apoptotic cell death induced by 5-aminolevulinic acid-mediated photodynamic actions in adult T-cell leukemia/lymphoma (ATL) cells (2012)
  • Author(s): Takashi Oka, Hirofumi Fujita, Lamia Abd Al-Kader, Hiaki Sato, Ichiro Murakami, Atae Utsunomiya and Tadashi Yoshino
  • Organizer: The 71st Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Sapporo
• [Presentation] 骨芽細胞分化培養に伴う石灰化におけるネクローシス細胞と活性酸素の関与 (2012)
  • Author(s): 藤田洋史、大内淑代、佐々木順造
  • Organizer: 第30回日本骨代謝学会
  • Place of Presentation: 東京
• [Presentation] 骨芽細胞分化培養に伴う石灰化と活性酸素の関与 (2012)
  • Author(s): 藤田洋史、大本苗起子、荻野哲也、佐々木順造、内海耕慥、大内淑代
  • Organizer: 第65回日本酸化ストレス学会学術集会
  • Place of Presentation: 徳島
• [Patent(Industrial Property Rights)] METHOD USING ABNORMALLY-ACTIVATED-CELL DETECTION TO TEST FOR MALIGNANT TUMORS AND ABNORMALLY-ACTIVATED-CELL APHERESIS-THERAPY APPARATUS (2014)
  • Inventor(s): 岡剛史、藤田洋史、吉野正
  • Industrial Property Rights Holder: 岡剛史、藤田洋史、吉野正
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-06-20
  • Overseas
• [Patent(Industrial Property Rights)] 異常活性化白血球除去還流返血治療装置 (2013)
  • Inventor(s): 岡 剛史、藤田 洋史
  • Industrial Property Rights Holder: 岡 剛史、藤田 洋史
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-130758
  • Filing Date: 2013-06-21