The role of small GTP-binding proteins and cytoskeleton in the development of osteoarthritis and chondrogenesis
| Project/Area Number |
24592267
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
MATSUDA Shuichi 京都大学, 医学(系)研究科(研究院), 教授 (40294938)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | スタチン / 低分量G蛋白 / 軟骨変性 / 軟骨再生 |
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| Outline of Final Research Achievements |
The purpose of this study was to investigate the role of small GTP-binding proteins and cytoskeleton in the development of osteoarthritis and chondrogenesis. Human chondrocytes and synoviocytes were transfected with siRNA for RhoA, Rac1, and CDC42. Knock-down of small GTP-binding proteins resulted in the induction of chondrocyte specific gene expressions in chondrocytes as well as the reduction of proinflammatory gene expression in synoviocytes. Especially, the effects of Rac1 knock-down were consistent with those of statin on the expressions of chondrocyte specific genes and proinflammatory genes.
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Report
(4 results)
Research Products
(14 results)
