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Transcriptional regulation of a novel collagen gene in chondrocytes

Research Project

Project/Area Number 24592270
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionOita University

Principal Investigator

Matsuo Noritaka  大分大学, 医学部, 准教授 (10284788)

Co-Investigator(Kenkyū-buntansha) YOSHIOKA Hidekatsu  大分大学, 医学部, 教授 (00222430)
SASAKI Takako  大分大学, 医学部, 助教 (30133193)
OKAMOTO Osamu  大分大学, 医学部, 客員研究員 (40284799)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords軟骨 / 転写 / コラーゲン
Outline of Final Research Achievements

We investigated the proximal promoter of mouse Col11a1 and Col27a1 gene in chondrocytes.In Col27a1 gene, oligo-Capping Race analysis revealed that mouse Col27a1 gene has two alternative splicing variants in chondrocytes. Transient transfection experiments indicated that the proximal promoter activity was from -586 to -445 in the downstream promoter, and was from -310 to +1 in the upstream promoter. In Col11a1 gene, cell transfection experiments exhibited the suppression of the promoter activity without NF-Y binding sequence. Furthermore, dominant-negative NF-Y inhibited the promoter activity. In addition, luciferase assays exhibited that GC rich sequence is a critical elements. Overexpression of Sp1 was significantly increased, and knockdown of Sp1 was suppressed the expression of endogenous transcript of Col11a1 gene. Taken together, these results indicate that the transcription factor NF-Y and Sp1 upregulates the proximal promoter activity of mouse Col11a1 gene in chondrocytes.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (11 results)

All 2016 2015 2014 2012

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 5 results) Presentation (2 results)

  • [Journal Article] 1)Sp1 upregulated the proximal promoter activity of the mouse collagen a1(XI) gene (Col11a1) in chondrocytes.2016

    • Author(s)
      Watanabe K, Hida M, Sasaki T, Yana H, Kawano K, Yoshioka H, Matsuo N.
    • Journal Title

      In Vitro Cell Dev Biol Anim

      Volume: 52 Issue: 2 Pages: 235-42

    • DOI

      10.1007/s11626-015-9959-y

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sp7/Osterix induces the mouse pro-α2(I) collagen gene (Col1a2) expression via the proximal promoter in osteoblastic cells.2014

    • Author(s)
      Yano H, Hamanaka R, Nakamura-Ota M, Adachi S, Zhang JJ, Matsuo N, Yoshioka H.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 452 Issue: 3 Pages: 531-6

    • DOI

      10.1016/j.bbrc.2014.08.100

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of fibronectin binding sites in dermatopontin and their biological function.2014

    • Author(s)
      Kato A, Okamoto O, Wu W, Matsuo N, Kumai J, Yamada Y, Katagiri F, Nomizu M, Fujiwara S.
    • Journal Title

      J Dermatol Sci.

      Volume: 76 Issue: 1 Pages: 51-9

    • DOI

      10.1016/j.jdermsci.2014.07.003

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional peptide of dermatopontin produces fibrinogen fibrils and modifies its biological activity.2014

    • Author(s)
      Wu W, Okamoto O, Kato A, Matsuo N, Kumai J, Nomizu M, Fujiwara S.
    • Journal Title

      J Dermatol Sci.

      Volume: 76 Issue: 1 Pages: 34-43

    • DOI

      10.1016/j.jdermsci.2014.07.002

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nuclear factor Y (NF-Y) regulates the proximal promoter activity of the mouse collagen α1(XI) gene (Col11a1) in chondrocytes.2014

    • Author(s)
      Hida M, Hamanaka R, Okamoto O, Yamashita K, Sasaki T, Yoshioka H, Matsuo N.
    • Journal Title

      In Vitro Cell Dev Biol Anim.

      Volume: 50 Pages: 358-66

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dermatopontin regulates fibrin formation and its biological activity2014

    • Author(s)
      Wu W, Okamoto O, Kato A, Matsuo N, Nomizu M, Yoshioka H, Fujiwara S.
    • Journal Title

      J Invest Dermatol.

      Volume: 134 Issue: 1 Pages: 256-263

    • DOI

      10.1038/jid.2013.305

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transient expression of mouse pro-α3(V) collagen gene (Col5a3) in wound healing.2012

    • Author(s)
      Sumiyoshi H, Kitamura H, Matsuo N, Tatsukawa S, Ishikawa K, Okamoto O, Fujikura Y, Fujiwara S, Yoshioka H.
    • Journal Title

      Connect Tissue Res.

      Volume: 53 Issue: 4 Pages: 313-7

    • DOI

      10.3109/03008207.2011.653061

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Smad, but not MAPK, pathway mediates the expression of type I collagen in radiation induced fibrosis.2012

    • Author(s)
      Yano H, Hamanaka R, Nakamura M, Sumiyoshi H, Matsuo N, Yoshioka H
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 418 Issue: 3 Pages: 457-63

    • DOI

      10.1016/j.bbrc.2012.01.039

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The PI3K/Akt pathway mediates the expression of type I collagen induced by TGF-b2 in human retinal pigment epithelial cells.2012

    • Author(s)
      Yokoyama K ,Kimoto K, Itoh Y, Nakatsuka K, Matsuo N, Yoshioka H, Kubota T
    • Journal Title

      Graefes Arch Clin Exp Ophthalmol,

      Volume: 250 Issue: 1 Pages: 15-23

    • DOI

      10.1007/s00417-011-1766-x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] XI型コラーゲンα1鎖遺伝子(Col11a1)の転写調節機構の解明2015

    • Author(s)
      渡邊啓次郎、樋田真理子、佐々木隆子、河野憲司、吉岡秀克、松尾哲孝
    • Organizer
      第39回蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      豊泉荘(大分県、別府市)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] マウスXXVII型コラーゲンα1鎖遺伝子の転写調節機構の解析2012

    • Author(s)
      樋田真理子、松尾哲孝、山下広平、足立佐和子、佐々木隆子、吉岡秀克
    • Organizer
      日本分子生物学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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