| Project/Area Number |
24592273
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagoya City University |
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Principal Investigator |
FUKUOKA Muneyoshi 名古屋市立大学, 医学(系)研究科(研究院), 講師 (80285204)
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| Co-Investigator(Kenkyū-buntansha) |
MIZUTANI Jun 名古屋市立大学, 大学院医学研究科, 講師 (70326156)
OTSUKA Takanobu 名古屋市立大学, 大学院医学研究科, 教授 (10185316)
KOZAWA Osamu 岐阜大学, 大学院医学研究科, 教授 (90225417)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 骨芽細胞 / HSP27 / 骨代謝 / 骨粗鬆症 / オステオカルシン / eIF4E / 翻訳 / ＨＳＰ27 / リン酸化 / Osteoprotegerin / Osteocalcin / VEGF / FGF-2 |
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| Outline of Final Research Achievements |
Heat shock proteins (HSPs) are induced by a variety of physiological and environmental stresses, such as heat. As molecular chaperones, HSPs facilitate the refolding of unfolded proteins. However, the details behind the HSP27-mediated effects on osteoblasts remain to be clarified. In the present study, in order to investigate the exact mechanism of HSP27 and its phosphorylation in osteoblasts, we explored the molecular targets of HSP27 using osteoblast-like MC3T3-E1 cells. Our results strongly suggest that the phosphorylation status of HSP27 play a role in switching its binding to eIF4E, resulting in regulation of the translation initiation process in osteoblasts.
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