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Circulating nucleated peripheral blood cells contribute to early-phase meniscal healing

Research Project

Project/Area Number 24592275
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionOsaka City University

Principal Investigator

MINODA Yukihide  大阪市立大学, 医学(系)研究科(研究院), 講師 (90453133)

Co-Investigator(Kenkyū-buntansha) HASHIMOTO Yusuke  大阪市立大学, 医学(系)研究科(研究院), 講師 (10382178)
NAKAMURA Hiroaki  大阪市立大学, 医学(系)研究科(研究院), 教授 (60227931)
IWAKI Hiroyoshi  大阪市立大学, 医学(系)研究科(研究院), 准教授 (20381981)
WAKITANI Shigeyuki  武庫川女子大学, スポーツ健康科学部, 教授 (70243243)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords半月修復 / パラバイオシス / 血流由来細胞 / マクロファージ / 半月板 / 修復
Outline of Final Research Achievements

The purpose of this study was to assess how peripheral blood cells (PBCs) contribute to meniscus repair, using a parabiotic rat model. the anterior part of the medial meniscus of parabiotic rat was removed. At 1, 2, 4, 8 and 12 weeks post-meniscectomy, repaired tissue was evaluated using stereomicroscopy. The ratios of PBCs in the repaired meniscus were 20.5% at 1 week, 8.3% at 2 weeks, 4.4% at 4 weeks, 2.1% at 8 weeks, post-meniscectomy. Histologically, fibrochondrocytes were observed in the repaired meniscus of WT rats after 4 weeks, some of which were GFP-positive. The chondrogenic marker, type II collagen, was merged within the PBCs in the repaired tissue. However, type-II-collagen-positive cell ratio and metachromasia in the repaired meniscus were not equivalent in normal meniscal tissue. This indicated that PBCs were present within the repaired meniscus at an early phase, replacing the excised meniscal cells, suggesting PBCs contributed to meniscal healing.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (19 results)

All 2015 2014 2013 2012 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (10 results)

  • [Journal Article] Small Periprosthetic Bone Defects after Total Knee Arthroplasty.2014

    • Author(s)
      Minoda Y, Yoshida T, Sugimoto K, Baba S, Ikebuchi M, Nakamura H.
    • Journal Title

      J Arthroplasty

      Volume: 29 Pages: 2280-4

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intraoperative assessment of midflexion laxity in total knee prosthesis.2014

    • Author(s)
      Minoda Y, Nakagawa S, Sugama R, Ikawa T, Noguchi T, Hirakawa M, Nakamura H.
    • Journal Title

      Knee.

      Volume: 21 Pages: 810-4

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Joint gap measurement in total knee arthroplasty using a tensor device with the same articulating surface as the prosthesis.2014

    • Author(s)
      Matsui Y, Nakagawa S, Minoda Y, Mizokawa S, Tokuhara Y, Kadoya Y
    • Journal Title

      Arch Orthop Trauma Surg.

      Volume: 134 Pages: 699-705

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] No differe nce in in vivo polyethylene wear particles between oxidized zirconium and cobalt-chrom ium femoral component in total knee arthroplasty.2014

    • Author(s)
      Minoda Y, Hata K, Iwaki H, Ikebuchi M, Hashimoto Y, Inori F, Nakamura H
    • Journal Title

      Knee Surg Sports Traumatol Arthrosc.

      Volume: 22 Pages: 680-6

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Porous tantalum tibial component prevents periprothetic loss of bone mineral density after total knee arthroplasty for five years -a matched cohort study-2013

    • Author(s)
      Minoda Y, Kobayashi A, Ikebuchi M, Iwaki H, Inori H, Nakamura H
    • Journal Title

      Journal of Arthroplasty

      Volume: 28 Issue: 10 Pages: 1764-1764

    • DOI

      10.1016/j.arth.2013.03.031

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Theoretical risk of anterior femoral cortex notching in total knee arthroplasty using a navigation system.2013

    • Author(s)
      Minoda Y, Watanabe K, Iwaki H, Takahashi S, Fukui M, Nakamura H
    • Journal Title

      Journal of Arthroplasty

      Volume: 28 Issue: 9 Pages: 1537-1537

    • DOI

      10.1016/j.arth.2013.02.015

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The flexion gap preparation does not disturb the modified gap technique in posterior stabilized total knee arthroplasty.2012

    • Author(s)
      Minoda Y, Watanabe K, Iwaki H, Takahashi S, Fukui M, Nakamura H.
    • Journal Title

      Knee

      Volume: 20(12) Pages: 2454-9

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mid-term clinical results of alumina medial pivot total knee arthroplasty. pivot total knee arthroplasty.2012

    • Author(s)
      Iida T, Minoda Y, Kadoya Y, Matsui Y, Kobayashi A, Iwaki H, Ikebuchi M, Yoshida T, Nakamura H.
    • Journal Title

      Knee Surg Sports Traumatol Arthrosc.

      Volume: 20(8) Pages: 1514-9

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 6. Early biological fixation of porous implant coated with paste-retaining recombinant bone morphogenetic protein 2.2012

    • Author(s)
      Fukunaga K, Minoda Y, Iwakiri K, Iwaki H, Nakamura H, Takaoka K
    • Journal Title

      J Arthroplasty.

      Volume: 27(1) Pages: 143-149

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Assymentric Tibial Component Reduced The Risk Of Malrotation In TKA2015

    • Author(s)
      Minoda Y, Mizokawa S, Ohta Y, Ikebuchi M, Itokazu M, Yamaura K, Nakamura S, Nakamura H
    • Organizer
      Annual meeting of American Academy of Orthopaedic Surgeons
    • Place of Presentation
      Las Vegas (USA)
    • Year and Date
      2015-03-24 – 2015-03-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] パラバイオーシスラットモデルを用いた半月修復におけるcirculation cellの関与の検討2013

    • Author(s)
      山崎 真哉, 橋本 祐介, 中村 博亮
    • Organizer
      第28回日本整形外科学会基礎学術集会
    • Place of Presentation
      千葉 幕張メッセ
    • Related Report
      2013 Research-status Report
  • [Presentation]  A Novel Technique of Tomography Detected Small Periprosthetic Bone Defects In TKA2013

    • Author(s)
      Minoda Y, Iwaki H, Yoshida T, Ikebuchi M, Mizokawa S, Sugimoto K, baba S, Kasai A, Nakamura H
    • Organizer
      Annual meeting of American Academy of Orthopaedic Surgeons
    • Place of Presentation
      McCormick Place, Chicago,USA
    • Related Report
      2012 Research-status Report
  • [Presentation]  Intra-Operative Assessment Of Mid-Flexion Instability In Total Knee Arthropalsty2013

    • Author(s)
      Minoda Y, Nakagawa S, Kobayashi A, Kadoya Y, Tomari K, Kondo M, Sugama R, Noguchi T, Higuma Y
    • Organizer
      Annual meeting of American Academy of Orthopaedic Surgeons
    • Place of Presentation
      McCormick Place, Chicago,USA
    • Related Report
      2012 Research-status Report
  • [Presentation]  Peri-prosthetic Bone Mineral Density after Bilateral Total Knee Arthroplasty Under Oral Alendronate Therapy2013

    • Author(s)
      Minoda Y, Ra K, Iwaki H, Ikebuchi M, Mizokawa S, Yoshida T, Nakamura H
    • Organizer
      Annual meeting of American Academy of Orthopaedic Surgeons
    • Place of Presentation
      McCormick Place, Chicago,USA
    • Related Report
      2012 Research-status Report
  • [Presentation]  Comparison Of In Vivo Wear Particles Between Sequentially Annealed HXLPE And Conventional PE In TKA2013

    • Author(s)
      Minoda Y, Hata K, Iwaki H, Ikebuchi M, Yoshida T, Mizokawa S, Nakamura H
    • Organizer
      Annual meeting of American Academy of Orthopaedic Surgeons
    • Place of Presentation
      McCormick Place, Chicago,USA
    • Related Report
      2012 Research-status Report
  • [Presentation] The Detection of Small Periprosthetic Bone Defects in Total Knee Arthroplasty Using A Novel Technique of Tomography -Tomosynthesis-2012

    • Author(s)
      Minoda Y, Iwaki H, Yoshida T, Ikebuchi M, Mizokawa S, Inori F, Itokazu M, Maki T, Sugimoto K, Baba S, Kasai A, Nakamura H.
    • Organizer
      25th Annual meeting of the International Society for Technology in Arthroplasty
    • Place of Presentation
      Hilton Sydney, Australia
    • Related Report
      2012 Research-status Report
  • [Presentation] Nucleated cells circulating in the peripheral blood Contribute to Meniscus Healing during mainly Early-Phase

    • Author(s)
      Shinya Yamasaki, Yusuke Hashimoto, Junsei Takigami, Shozaburo Terai, Masafumi Takahashi, Shigeyuki Wakitani, Hiroaki Nakamura
    • Organizer
      60th Annual meeting of Orthopaedic Research Society
    • Place of Presentation
      Hyatt Regency New Olreans,New Olreans,USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Sequentially Annealed Highly Cross-linked Polyethylene Reduced In Vivo Wear Particle Generation In TKA

    • Author(s)
      Minoda Y, Hata K, Mizokawa S, Ohta Y, Itokazu M, Yamamura K, Nakamura H
    • Organizer
      60th Annual meeting of Orthopaedic Research Society
    • Place of Presentation
      New Olreans Moiral Covention Center New Olreans,USA
    • Related Report
      2013 Research-status Report
  • [Presentation] The Detection of Small Periprosthetic Bone Defects in TKA using a Novel Technique of Tomography

    • Author(s)
      Minoda Y, Mizokawa S, Ikebuchi M, Ohta Y, Itokazu M, Yamamura K, Sugimoto K, Baba S, Kasai A, Nakamura H.
    • Organizer
      60th Annual meeting of Orthopaedic Research Society
    • Place of Presentation
      New Olreans Moiral Covention Center New Olreans,USA
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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