Circulating nucleated peripheral blood cells contribute to early-phase meniscal healing

• Project/Area Number: 24592275
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Osaka City University
• Principal Investigator: MINODA Yukihide 大阪市立大学, 医学(系)研究科(研究院), 講師 (90453133)
• Co-Investigator(Kenkyū-buntansha): HASHIMOTO Yusuke 大阪市立大学, 医学(系)研究科(研究院), 講師 (10382178) ; NAKAMURA Hiroaki 大阪市立大学, 医学(系)研究科(研究院), 教授 (60227931) ; IWAKI Hiroyoshi 大阪市立大学, 医学(系)研究科(研究院), 准教授 (20381981) ; WAKITANI Shigeyuki 武庫川女子大学, スポーツ健康科学部, 教授 (70243243)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 半月修復 / パラバイオシス / 血流由来細胞 / マクロファージ / 半月板 / 修復

Outline of Final Research Achievements

The purpose of this study was to assess how peripheral blood cells (PBCs) contribute to meniscus repair, using a parabiotic rat model. the anterior part of the medial meniscus of parabiotic rat was removed. At 1, 2, 4, 8 and 12 weeks post-meniscectomy, repaired tissue was evaluated using stereomicroscopy. The ratios of PBCs in the repaired meniscus were 20.5% at 1 week, 8.3% at 2 weeks, 4.4% at 4 weeks, 2.1% at 8 weeks, post-meniscectomy. Histologically, fibrochondrocytes were observed in the repaired meniscus of WT rats after 4 weeks, some of which were GFP-positive. The chondrogenic marker, type II collagen, was merged within the PBCs in the repaired tissue. However, type-II-collagen-positive cell ratio and metachromasia in the repaired meniscus were not equivalent in normal meniscal tissue. This indicated that PBCs were present within the repaired meniscus at an early phase, replacing the excised meniscal cells, suggesting PBCs contributed to meniscal healing.

Research Products

• [Journal Article] Small Periprosthetic Bone Defects after Total Knee Arthroplasty. (2014)
  • Author(s): Minoda Y, Yoshida T, Sugimoto K, Baba S, Ikebuchi M, Nakamura H.
  • Journal Title: J Arthroplasty Volume: 29 Pages: 2280-4
  • Peer Reviewed
• [Journal Article] Intraoperative assessment of midflexion laxity in total knee prosthesis. (2014)
  • Author(s): Minoda Y, Nakagawa S, Sugama R, Ikawa T, Noguchi T, Hirakawa M, Nakamura H.
  • Journal Title: Knee. Volume: 21 Pages: 810-4
  • Peer Reviewed
• [Journal Article] Joint gap measurement in total knee arthroplasty using a tensor device with the same articulating surface as the prosthesis. (2014)
  • Author(s): Matsui Y, Nakagawa S, Minoda Y, Mizokawa S, Tokuhara Y, Kadoya Y
  • Journal Title: Arch Orthop Trauma Surg. Volume: 134 Pages: 699-705
  • Peer Reviewed
• [Journal Article] No differe nce in in vivo polyethylene wear particles between oxidized zirconium and cobalt-chrom ium femoral component in total knee arthroplasty. (2014)
  • Author(s): Minoda Y, Hata K, Iwaki H, Ikebuchi M, Hashimoto Y, Inori F, Nakamura H
  • Journal Title: Knee Surg Sports Traumatol Arthrosc. Volume: 22 Pages: 680-6
  • Peer Reviewed
• [Journal Article] Porous tantalum tibial component prevents periprothetic loss of bone mineral density after total knee arthroplasty for five years -a matched cohort study- (2013)
  • Author(s): Minoda Y, Kobayashi A, Ikebuchi M, Iwaki H, Inori H, Nakamura H
  • Journal Title: Journal of Arthroplasty Volume: 28 Issue: 10 Pages: 1764-1764
  • DOI: 10.1016/j.arth.2013.03.031
  • Peer Reviewed
• [Journal Article] Theoretical risk of anterior femoral cortex notching in total knee arthroplasty using a navigation system. (2013)
  • Author(s): Minoda Y, Watanabe K, Iwaki H, Takahashi S, Fukui M, Nakamura H
  • Journal Title: Journal of Arthroplasty Volume: 28 Issue: 9 Pages: 1537-1537
  • DOI: 10.1016/j.arth.2013.02.015
  • Peer Reviewed
• [Journal Article] The flexion gap preparation does not disturb the modified gap technique in posterior stabilized total knee arthroplasty. (2012)
  • Author(s): Minoda Y, Watanabe K, Iwaki H, Takahashi S, Fukui M, Nakamura H.
  • Journal Title: Knee Volume: 20(12) Pages: 2454-9
  • Peer Reviewed
• [Journal Article] Mid-term clinical results of alumina medial pivot total knee arthroplasty. pivot total knee arthroplasty. (2012)
  • Author(s): Iida T, Minoda Y, Kadoya Y, Matsui Y, Kobayashi A, Iwaki H, Ikebuchi M, Yoshida T, Nakamura H.
  • Journal Title: Knee Surg Sports Traumatol Arthrosc. Volume: 20(8) Pages: 1514-9
  • Peer Reviewed
• [Journal Article] 6. Early biological fixation of porous implant coated with paste-retaining recombinant bone morphogenetic protein 2. (2012)
  • Author(s): Fukunaga K, Minoda Y, Iwakiri K, Iwaki H, Nakamura H, Takaoka K
  • Journal Title: J Arthroplasty. Volume: 27(1) Pages: 143-149
  • Peer Reviewed
• [Presentation] Assymentric Tibial Component Reduced The Risk Of Malrotation In TKA (2015)
  • Author(s): Minoda Y, Mizokawa S, Ohta Y, Ikebuchi M, Itokazu M, Yamaura K, Nakamura S, Nakamura H
  • Organizer: Annual meeting of American Academy of Orthopaedic Surgeons
  • Place of Presentation: Las Vegas （USA）
  • Year and Date: 2015-03-24 – 2015-03-26
• [Presentation] パラバイオーシスラットモデルを用いた半月修復におけるcirculation cellの関与の検討 (2013)
  • Author(s): 山崎 真哉, 橋本 祐介, 中村 博亮
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉 幕張メッセ
• [Presentation]  A Novel Technique of Tomography Detected Small Periprosthetic Bone Defects In TKA (2013)
  • Author(s): Minoda Y, Iwaki H, Yoshida T, Ikebuchi M, Mizokawa S, Sugimoto K, baba S, Kasai A, Nakamura H
  • Organizer: Annual meeting of American Academy of Orthopaedic Surgeons
  • Place of Presentation: McCormick Place, Chicago,USA
• [Presentation]  Intra-Operative Assessment Of Mid-Flexion Instability In Total Knee Arthropalsty (2013)
  • Author(s): Minoda Y, Nakagawa S, Kobayashi A, Kadoya Y, Tomari K, Kondo M, Sugama R, Noguchi T, Higuma Y
  • Organizer: Annual meeting of American Academy of Orthopaedic Surgeons
  • Place of Presentation: McCormick Place, Chicago,USA
• [Presentation]  Peri-prosthetic Bone Mineral Density after Bilateral Total Knee Arthroplasty Under Oral Alendronate Therapy (2013)
  • Author(s): Minoda Y, Ra K, Iwaki H, Ikebuchi M, Mizokawa S, Yoshida T, Nakamura H
  • Organizer: Annual meeting of American Academy of Orthopaedic Surgeons
  • Place of Presentation: McCormick Place, Chicago,USA
• [Presentation]  Comparison Of In Vivo Wear Particles Between Sequentially Annealed HXLPE And Conventional PE In TKA (2013)
  • Author(s): Minoda Y, Hata K, Iwaki H, Ikebuchi M, Yoshida T, Mizokawa S, Nakamura H
  • Organizer: Annual meeting of American Academy of Orthopaedic Surgeons
  • Place of Presentation: McCormick Place, Chicago,USA
• [Presentation] The Detection of Small Periprosthetic Bone Defects in Total Knee Arthroplasty Using A Novel Technique of Tomography -Tomosynthesis- (2012)
  • Author(s): Minoda Y, Iwaki H, Yoshida T, Ikebuchi M, Mizokawa S, Inori F, Itokazu M, Maki T, Sugimoto K, Baba S, Kasai A, Nakamura H.
  • Organizer: 25th Annual meeting of the International Society for Technology in Arthroplasty
  • Place of Presentation: Hilton Sydney, Australia
• [Presentation] Nucleated cells circulating in the peripheral blood Contribute to Meniscus Healing during mainly Early-Phase
  • Author(s): Shinya Yamasaki, Yusuke Hashimoto, Junsei Takigami, Shozaburo Terai, Masafumi Takahashi, Shigeyuki Wakitani, Hiroaki Nakamura
  • Organizer: 60th Annual meeting of Orthopaedic Research Society
  • Place of Presentation: Hyatt Regency New Olreans,New Olreans,USA
• [Presentation] Sequentially Annealed Highly Cross-linked Polyethylene Reduced In Vivo Wear Particle Generation In TKA
  • Author(s): Minoda Y, Hata K, Mizokawa S, Ohta Y, Itokazu M, Yamamura K, Nakamura H
  • Organizer: 60th Annual meeting of Orthopaedic Research Society
  • Place of Presentation: New Olreans Moiral Covention Center New Olreans,USA
• [Presentation] The Detection of Small Periprosthetic Bone Defects in TKA using a Novel Technique of Tomography
  • Author(s): Minoda Y, Mizokawa S, Ikebuchi M, Ohta Y, Itokazu M, Yamamura K, Sugimoto K, Baba S, Kasai A, Nakamura H.
  • Organizer: 60th Annual meeting of Orthopaedic Research Society
  • Place of Presentation: New Olreans Moiral Covention Center New Olreans,USA