Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Outline of Final Research Achievements |
IL-32 is known to induce various inflammatory cytokines and trigger the TLR signaling cascade, however, receptors and downstream signaling pathways remain to be clarified. We found that IL-32 upregulated PR3, in turn triggering PAR2 signaling. TNFα and typeⅠ interferon expression decreased with siRNA targeting TRIF. Initially IL-32-PAR2-TRIF pathway induce TNFα expression and latter pathway induces typeⅠ interferon expression via IRF. IL-32 signaling gradually increases typeⅠ interferon expression to translate innate to adaptive immunity and terminate acute inflammation . IL-32-PAR2-TRIF axis may have been gained during the evolution of mammalian immune systems in order to function not only as an extracellular sensor of bacterial and autologous proteases, but also as an interface between innate and adaptive immunity.
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