| Project/Area Number |
24592289
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
OHNISHI Hideo 産業医科大学, 医学部, 非常勤医師 (20279342)
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| Co-Investigator(Kenkyū-buntansha) |
UETA Yoichi 産業医科大学, 医学部, 教授 (10232745)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Toshitaka 国立国際医療研究センター病院, 院長 (50082235)
MORI Toshiharu 産業医科大学, 医学部, 講師 (80525444)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 疼痛 / c-fos遺伝子 / トランスジェニック動物 / 光遺伝学 / 蛍光タンパク |
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| Outline of Final Research Achievements |
We have generated a novel transgenic rat system that enables the visualization of c-fos expression, using an enhanced green fluorescent protein (eGFP) tag (c-fos-eGFP transgenic rat). Furthermore, we have generated rats bearing an c-fos-channelrhodopsin 2 (ChR2) -eGFP fusion transgene (c-fos-ChR2-eGFP transgenic rat). The purposes of the study were to visualize the mechanism of acceptance against nociceptive stress and to try operating the neural activities of Fos-ChR2 expressing neurons by light irradiation, using these transgenic rats. We succeeded in visualizing the c-fos-eGFP expression in the hypothalamus, spinal cord, anterior pituitary, and adrenal cortex after nociceptive stress in in vitro or ex vivo in c-fos-eGFP transgenic rats. In addition, ChR2-eGFP expression on the cell menbrance in the hypothalamus and the spinal cord was observed after nociceptive stress in c-fos-ChR2-eGFP transgenic rats.
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