| Project/Area Number |
24592297
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HAMADA Hiroshi 広島大学, 大学院医歯薬保健学研究院, 准教授 (10218539)
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| Research Collaborator |
MUKAIDA Keiko 広島県障害者リハビリテーションセンター, 麻酔科, 主任医長
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 悪性高熱症 / 骨格筋細胞 / カルシウムイオン / ダントロレン / HEK細胞 / リアノジン受容体 / 悪性高熱 / カルシウム濃度 / スキンドファイバー / ヒト培養骨格筋 / カルシウム動態 |
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| Outline of Final Research Achievements |
This study aimed to investigate the inhibitory effects of dantrolene on RYR1 sensitivity related to Ca2+ release in cultured myotubes obtained from MH (Malignant hyperthermia)-predisposed patients and assessed the possibility of preventing MH crisis with dantrolene pretreatment. The Ca2+ in each myotubes from MH-predisposed patients were determined by using the fluorescent Ca indicator fura-2, and the effect of dantrolene in lowering Ca2+ were examined. Caffeine was used to activate RYR1 and half-maximal activation concentrations (EC50) of caffeine were determined in myotubes. Using 15 microM dantrolene increased the EC50 for caffeine near the normal range in myotubes. Pretreatment with 15 microM of dantrolene can prevent MH crises.
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