| Project/Area Number |
24592300
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | The University of Tokushima |
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Principal Investigator |
OSHITA Shuzo 徳島大学, ヘルスバイオサイエンス研究部, 非常勤講師 (60144945)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Katsuya 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授 (30263841)
TSUTSUMI Yasuo 徳島大学, 大学院ヘルスバイオサイエンス研究部, 准教授 (90523499)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | GLP-1受容体 / 虚血再潅流障害 / 吸入麻酔薬 / イソフルラン / GLP-1 / 心保護作用 / 虚血再灌流障害 |
|---|
| Outline of Final Research Achievements |
Exendin-4, GLP-1 receptor agonist, protected cardiac myocytes (CM) from ischemia-reperfusion. Additionally, myocardial infarct size was smaller in the isoflurane- [1.0 minimum alveolar concentration (MAC)] or exendin-4- (30 ng/kg i.v.) treated groups than the controls. Infarct size was not affected by isoflurane at 0.5 MAC or 3 ng/kg i.v. exendin-4, but the combination of these treatments reduced infarct size.
Immunofluorescence analysis of the expression and colocalization of caveolin-3 (Cav-3) and GLP-1R in cardiac myocytes. Fluorescent secondary antibodies were used to determine Cav-3 and GLP-1R localization, and strong colocalization were observed on the cell surface membrane. Additionally, these preconditioning significantly attenuated the calcium-induced swelling compared with the control.
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