Anesthetic effect on dynamics of membrane microdomains in living cells

Research Project

Project/Area Number	24592301
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Anesthesiology/Resuscitation studies
Research Institution	Kagawa University
Principal Investigator	ONO Junichiro 香川大学, 医学部附属病院, その他 (90363217)
Co-Investigator(Renkei-kenkyūsha)	SUZUKI Shingo 香川大学, 医学部・神経機能形態学, 助教 (50451430)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000) Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Keywords	マイクロドメイン / 揮発性麻酔薬 / 麻酔メカニズム / FRAP解析 / TIRFM解析 / 脂質二重膜 / FRAP / 静脈麻酔薬 / GABAA受容体 / PRIP
Outline of Final Research Achievements	Lipid rafts are membrane microdomains which abundantly contain sphingolipids, sterols, and receptor proteins. Lipid rafts has crucial role in the signal transduction. Volatile anesthetics with high affinity to lipid distribute in the lipid bilayer. Therefore, it is likely that lipid rafts is target of volatile anesthetic action. The objective of this study is to determine whether the dynamics of lipid raft is affected by volatile anesthetics.