| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Outline of Final Research Achievements |
The analgesic effects of nitrous oxide(N2O) were investigated using tail flick test in Wistar rats received cannabinoid 1(CB1) receptor antagonist, rimonabant. In the rats injected rimonabant intrathecally, the percent of maximal possible effect(%MPE) did not increase with N2O inhalation. However, in the group administered rimonabant intraperitoneally and intraventricularly, %MPE did not decrease from the peak level. The anandamide might interact with N2O in spinal level.
RT-PCR assays demonstrated that CB1 receptor expression in rat reduced after 2 hours of exposure of N2O. CB1 receptor is known to have decreased its expression when exposed to a ligand for a long time: desensitization. It is suggested that N2O itself indicate the cannabinoids-like action or reinforce the action of endogenous cannabinoids.
|
