Practical use with control of aquaporin for brain edema decrease using knockdown technique
| Project/Area Number |
24592310
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | Aichi Medical University (2014)
Nagoya City University (2012-2013) |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SOBUE Kazuya 名古屋市立大学大学院, 医学研究科, 教授 (90264738)
ASAI Kiyofumi 名古屋市立大学大学院, 医学研究科, 教授 (70212462)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | aquaporin / knockdown / hypothermia / アクアポリン / 脳浮腫 / ノックダウン / AQP4 |
|---|
| Outline of Final Research Achievements |
Using vector expressing shRNA, we constructed lentivirus expressing sh RNAiof aquaporin(AQP) 4. We confirmed AQP4 knockdown that was stronger than our earlier study. We constructed hAQP4-M23 and hAQP4-M1 vector and transfected into C6 cells. We confirmed the overexpression of hAQP4-M1 and hAQP4-M23 in C6 cells. Moreover, we constructed permanent cell line of hAQP4-M23-overexpressed-cells of C6.
|
Report
(4 results)
Research Products
(24 results)
-
[Journal Article] Estimation of the success rate of anesthetic management for thymectomy in patients with myasthenia gravis treated without muscle relaxants: A retrospective observational cohort study2015
Author(s)
Yoshihito Fujita, Satoru Moriyama, Satoshi Aoki, Saya Yoshizawa, Maiko Tomita, Taiki Kojima, Yukiko Mori, Naoko Takeuchi, Min-Hye So, Motoki Yano, Kazuya Sobue.
-
Journal Title
Jounal of Anesthesia
Volume: 29
Pages: 1-1
Related Report
Peer Reviewed
-
-
-
-
-
-
[Journal Article] A comparison between dosages and plasma concentrations of dexmedetomidine in clinically ill patients: a prospective, observational, cohort study in Japan2013
Author(s)
Yoshihito Fujita, Koichi Inoue, Tasuku Sakamoto, Saya Yoshizawa, Maiko Tomita, Yoshie Maeda, Hitomi Taka, Ai Muramatsu, Youichiro Hattori, Hiroyuki Hirate, Toshimasa Toyo’oka, Kazuya Sobue
-
Journal Title
Journal of Intensive Care
Volume: 1
Issue: 1
Pages: 15-15
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
