Mechanism elucidation of the general anesthesia from a view point of the changes in the internal gene expression

• Project/Area Number: 24592318
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Nippon Medical School
• Principal Investigator: SAKAMOTO Atsuhiro 日本医科大学, 医学(系)研究科(研究院), 教授 (30196084)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 全身麻酔 / 遺伝子発現 / microRNA / TLDA / 吸入麻酔薬 / 静脈麻酔薬 / マイクロRNA / 神経障害性疼痛 / マイクロＲＮＡ / 全身麻酔薬 / miRNA

Outline of Final Research Achievements

The effects of general anesthetics on the changes of internal gene expression, especially microRNA (miRNA) expression were evaluated mainly by TaqMan Low-density Array (TLDA) analysis. The main results are as follows. (1) The general anesthetics affect miRNA expression in the vital organs (lung, liver, blood, brain and supine). (2) The different anesthetics show the different influence on miRNA expression. (3) The pathologic changes of the central nervous system influence the expression of some miRNAs. These results provide the basic data of miRNA expression for the mechanism elucidation of the general anesthesia.

Research Products

• [Journal Article] Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia (2014)
  • Author(s): Goto G, Hori Y, Ishikawa M, Tanaka S, Sakamoto A
  • Journal Title: MolMed Rep Volume: 9 Issue: 5 Pages: 1715-1722
  • DOI: 10.3892/mmr.2014.2038
  • Peer Reviewed
• [Journal Article] Sevoflurane anesthesia persistently downregulates muscle-specific microRNAs in rat plasma (2014)
  • Author(s): Jumpai Takeuchi, Atsuhiro Sakamoto, Toshihiro Takizawa
  • Journal Title: Int J Mol Med Volume: 34 Issue: 1 Pages: 291-298
  • DOI: 10.3892/ijmm.2014.1739
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Epigenetic supresiion of mouse Per2 expression in the suprachiasmatic nucleus by the inhalation anesthetic, sevoflurane (2014)
  • Author(s): Mori K, Iijima N, Higo S, Aikawa S, Matsuo I, Takumi K, Sakamoto A, Ozawa H
  • Journal Title: PLoS One Volume: 9 Issue: 1 Pages: e87319-e87319
  • DOI: 10.1371/journal.pone.0087319
  • Peer Reviewed
• [Journal Article] Differential expression of rat hippocampal micro RNA in two rat models of choronic pain (2013)
  • Author(s): Hori Y, Goto G, Arai-Iwasaki M, Ishikawa M, Sakamoto A
  • Journal Title: Int J Mol Med Volume: 32 Issue: 6 Pages: 1287-1292
  • DOI: 10.3892/ijmm.2013.1504
  • Peer Reviewed
• [Journal Article] microRNA changes in the dorsal horn of the spinal cord of rats with chronic constriction injury: A TqMan low density array study. (2013)
  • Author(s): Genda Y, Arai M, Ishikawa M, Tanaka S, Okabe T, Sakamoto A
  • Journal Title: International Journal of Molecular Medicine Volume: 31 Issue: 1 Pages: 129-137
  • DOI: 10.3892/ijmm.2012.1163
  • Peer Reviewed
• [Journal Article] Differences in microRNA changes of healthy rat liver between sevoflurane and propofol anesthesia. (2012)
  • Author(s): Ishikawa M, Tanaka S, Arai M, Genda Y, Sakamoto A
  • Journal Title: Anesthesiology Volume: 117 Issue: 6 Pages: 1245-1252
  • DOI: 10.1097/aln.0b013e3182746676
  • Peer Reviewed
• [Journal Article] Changes in microRNA expression in rat lungs caused by sevoflurane anesthesia : a TaqMan low-density array study (2012)
  • Author(s): Tanaka S, Ishikawa M, Arai M, Genda Y, Sakamoto A
  • Journal Title: Biomedical Research Volume: 33 Pages: 256-263
  • Peer Reviewed
• [Presentation] Common microRNAs regulate liver protection of anesthetic and ischemic preconditioning (2014)
  • Author(s): Tomonori Morita, Masashi Ishikawa, Atsuhiro Sakamoto
  • Organizer: 2014 Annual meeting of American Society of Anesthesiologists
  • Place of Presentation: San Francisco (America)
  • Year and Date: 2014-10-13
• [Presentation] Changes of messenger RNA expression in the prerontal cortex of pain model rats by electroconvulsive stimulation (2014)
  • Author(s): Yusuke Kimura, Atsuhiro Sakamoto
  • Organizer: 2014 Annual meeting of American Society of Anesthesiologists
  • Place of Presentation: San Francisco (America)
  • Year and Date: 2014-10-11
• [Presentation] ラット急性肺障害モデルにおける麻酔薬の作用とmicroRNAの発現変化 (2014)
  • Author(s): 大槻達郎、石川真士、田中俊輔、保利陽子、後藤玄太郎、坂本篤裕
  • Organizer: 日本麻酔科学会第61回学術集会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-05-15
• [Presentation] Changes in blood volume and colloid osmotic pressure during fluid absorption in patients undergoing endoscopic urosurgery (2013)
  • Author(s): Yagi K, Kinugawa C, Sakamoto A
  • Organizer: The ANESTHESIOLOGY 2013 annual meeting
  • Place of Presentation: San Francisco
• [Presentation] Epigenetic regulation of period expression in the mouse suprachiasmatic nucleus by sevoflurane (2013)
  • Author(s): Mori K, Matsuo I, Higo S, Aikawa S, Anzai M, Iijima N, Ozawa H, Sakamoto A
  • Organizer: The ANESTHESIOLOGY 2013 annual meeting
  • Place of Presentation: San Francisco
• [Presentation] Sevoflurane ameliorates lipopolysaccharide-induced acute lung injury and changes microRNA expression (2013)
  • Author(s): Otsuki T, Ishikawa M, Tnaka S, Hori Y, Goto G, Sakamoto A
  • Organizer: The ANESTHESIOLOGY 2013 annual meeting
  • Place of Presentation: San Francisco
• [Presentation] Sevoflurane anesthesia downregulates circulation muscle-derived miRNA (2013)
  • Author(s): Takeuchi J, Takizawa T, Sakamoto A
  • Organizer: The ANESTHESIOLOGY 2013 annual meeting
  • Place of Presentation: San Francisco
• [Presentation] Chronic constriction injury rat脊髄後角のmicroRNA変化の検討ーTaqMan Loe Density Arrayを用いたmicroRNA網羅解析ー (2012)
  • Author(s): 源田雄紀、荒井雅江、石川真士、田中俊輔、岡部格、坂本篤裕
  • Organizer: 第59回日本麻酔科学会学術集会
  • Place of Presentation: 神戸市
• [Presentation] ラット肝臓microRNA発現に対するセボフルラン、プロポフォールの影響 (2012)
  • Author(s): 石川真士、田中俊輔、荒井雅江、源田雄紀、坂本篤裕
  • Organizer: 第59回日本麻酔科学会学術集会
  • Place of Presentation: 神戸市
• [Presentation] Changes in gene expression of micromas on rats hippocampus (2012)
  • Author(s): Goto G, Ishikawa M, Skamoto A
  • Organizer: Annual Meeting of American Society of Anesthesiologists 2012
  • Place of Presentation: Washington DC
• [Presentation] Differential expression of hippocampal micromas in rat chronic pain models (2012)
  • Author(s): Hori Y, Arai M, Ishikawa M, Genda Y, Tanaka S, Sakamoto A
  • Organizer: Annual Meeting of American Society of Anesthesiologists 2012
  • Place of Presentation: Washington DC