The study of the trigeminal neuralgia occurrence mechanism which an aberrant joining between cells in the ganglion causes

• Project/Area Number: 24592319
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Kansai University of Welfare Sciences (2013-2014); Osaka Medical College (2012)
• Principal Investigator: HAYASAKI HANA 関西福祉科学大学, 保健医療学部, 准教授 (90257866)
• Co-Investigator(Kenkyū-buntansha): FUKUDA Astuo 浜松医科大, 医学部, 教授 (50254272) ; 相馬 義郎 慶應義塾大学, 医学部, 准教授 (60268183)
• Co-Investigator(Renkei-kenkyūsha): SOHMA Yoshiro 慶応大学, 医学部, 准教授 (60268183)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: GABA / 三叉神経節 / satellite cell / 三叉神経痛 / KCTD / GABA受容体 / GABAB1 / GABAB2 / KCTD12 / KCTD16 / ラット / GABAB / GABA receptor / trigeminal ganglion

Outline of Final Research Achievements

We investigated GABA system with the trigeminal ganglion with the possibility to contribute to trigeminal neuralgia.We found heterogeneous expressions of GABABR1 and GABABR2 subunits and auxiliary subunits KCTD12 and KCTD16 in the rat trigeminal ganglion (TG).
The spinal trigeminal nucleus caudalis (Sp5C) is a critical relay site for processing nociceptive afferent input from the orofacial area and for its modulation by neuroplastic changes.Axons originating from TG carry information from facial and cranial nociception into brainstem.After entering brainstem, forming the spinal trigeminal tract and terminate in Sp5C.In this study, interactions of GABABR1, GABABR2, KCTD12 and KCTD16 in TG and brainstem including Sp5C using co-immunoprecipitation assay and immnohistohemistry.

Research Products

• [Journal Article] 27．Inflammation as well as angiogenesis may participate in the pathophysiology of brain radiation necrosis. (2014)
  • Author(s): Yoritsune E1, Furuse M, Kuwabara H, Miyata T, Nonoguchi N, KawabataS, Hayasaki H, Kuroiwa T, Ono K, Shibayama Y, Miyatake SI.
  • Journal Title: J Radiat Res. Volume: doi:10.1093 Pages: 1-9
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Heterogenous GABA(B) receptor-mediated pathways are involved in the local GABAergic system of the rat trigeminal ganglion: possible involvement of KCTD proteins. (2012)
  • Author(s): Hayasaki H, Sohma Y, Kanbara K, Otsuki Y.
  • Journal Title: Neuroscience. Volume: 218 Pages: 344-358
  • DOI: 10.1016/j.neuroscience.2012.05.038
  • Peer Reviewed
• [Presentation] Expression and interaction of GABAB receptors and KCTD proteins in rat trigeminal ganglion and spinal trigeminal nucleus caudalis (2014)
  • Author(s): H. Hayasaki, Y. Otsuki, Y. Sohma
  • Organizer: 第３７回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-13
• [Presentation] ラット三叉神経節におけるGABAB レセプター応答の不均一性におけるKCTD サブユニットの寄与 (2013)
  • Author(s): Hayasaki H, Sohma Y, Kanbara K, Otsuki Y.
  • Organizer: 第118回日本解剖学会総会
  • Place of Presentation: サンポートホール高松（香川）
• [Presentation] Heterogeneous GABAB function underlied by KCTD Proteins in Rat Trigeminal Ganglion (2012)
  • Author(s): Hayasaki H, Sohma Y, Kanbara K, Otsuki Y.
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Book] PT OT STのための解剖学 (2013)
  • Author(s): 渡辺正仁監修 早﨑 華
  • Total Pages: 421
  • Publisher: 廣川書店