The impact of HIF-1 activation on respiratory epithelium during hypoxic insults.
| Project/Area Number |
24592322
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | The Tazuke Kofukai |
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Principal Investigator |
ADACHI Takehiko 公益財団法人田附興風会, 医学研究所 第9研究部, 部長 (90252428)
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| Co-Investigator(Kenkyū-buntansha) |
HIROTA Kiichi 関西医科大学, 医学部, 准教授 (00283606)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 低酸素誘導性因子1 / 無気肺 / タイトジャンクション / 肺障害 / HIF-1 / 肺水腫 / 肺胞炎症 / 低酸素 |
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| Outline of Final Research Achievements |
The respiratory epithelium forms an important barrier against inhaled pollutants and microorganisms, and its barrier function is often compromised during hypoxic insults. Epithelial activation of hypoxia-inducible factor-1 (HIF-1) represents one feature of airway inflammation, but the functional importance of HIF-1 within the respiratory epithelium is largely unknown. We evaluated the impact of HIF-1 activation on loss of epithelial barrier function during hypoxic insults. Involvement of HIF-1 in these protective effects was confirmed using the pharmacological inhibitor YC-1 and the activator of HIF-1 DFX adopting A549 cells derived from alveolar epithelium. Expression of mRNA and protein of an subunit of Namiloride-blockable ENaC, APQ5 and molecules involving in cell-cell tight junction ZO-1, occudin, claudin were investigated. The critical involvement of HIF-1 was demonstrated.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] UCHL1 provides diagnostic and antimetastatic strategies due to its deubiquitinating effect on HIF-1alpha2015
Author(s)
Goto Y, Zeng L, YeomCJ, Zhu Y, Morinibu A, Shinoyam K, Kobayashi M, Hirota K, Itasaka S, Yoshimura M, Tanimoto K, Sowa T, Menju T, Sonobe M, Kakeya H, Toi M, Date H, Hammond EM, Hiraoka M, Harada H.
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Journal Title
Nat Commun
Volume: 6
Issue: 1
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access
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