| Project/Area Number |
24592322
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | The Tazuke Kofukai |
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Principal Investigator |
ADACHI Takehiko 公益財団法人田附興風会, 医学研究所 第9研究部, 部長 (90252428)
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| Co-Investigator(Kenkyū-buntansha) |
HIROTA Kiichi 関西医科大学, 医学部, 准教授 (00283606)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 低酸素誘導性因子1 / 無気肺 / タイトジャンクション / 肺障害 / HIF-1 / 肺水腫 / 肺胞炎症 / 低酸素 |
|---|
| Outline of Final Research Achievements |
The respiratory epithelium forms an important barrier against inhaled pollutants and microorganisms, and its barrier function is often compromised during hypoxic insults. Epithelial activation of hypoxia-inducible factor-1 (HIF-1) represents one feature of airway inflammation, but the functional importance of HIF-1 within the respiratory epithelium is largely unknown. We evaluated the impact of HIF-1 activation on loss of epithelial barrier function during hypoxic insults. Involvement of HIF-1 in these protective effects was confirmed using the pharmacological inhibitor YC-1 and the activator of HIF-1 DFX adopting A549 cells derived from alveolar epithelium. Expression of mRNA and protein of an subunit of Namiloride-blockable ENaC, APQ5 and molecules involving in cell-cell tight junction ZO-1, occudin, claudin were investigated. The critical involvement of HIF-1 was demonstrated.
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