Analgesic mechanism of tetrabiopterin and cholinergic metabolism inhibitors
| Project/Area Number |
24592334
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | Shiga University of Medical Science |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
林 維光 , 医学部, 助教 (80242973)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | GTP cyclohydrolase 1 / dorsal root ganglion / アセチルコリン / tetrahydrobiopterin / 後根神経節 / 痛覚伝達 / テトラヒドロビオプテリン / Dorsal root ganglion / Brain Stem |
|---|
| Outline of Final Research Achievements |
GCH1 is the first and rate-limiting enzyme in the biosynthetic pathway of tetrahydrobiopterin (BH4), an important cofactor for the synthesis of several modulator in pain neurotransmission. Recently, the importance of GCH1 as pain marker has been recognized. The activity of GCH1 is regulated by its regulatory protein GTP cyclohydrolase regulatory protein (GFRP). Here we studied the regulation mechanism of GCH1/GFRP complexation using immunohistochemistry, proximity ligation assay and blue native electrophoresis. It was observed that GCH1 and GFRP were widely expressed in population of serotoninergic, dopaminergic and nitrinergic neurons, but GCH1/GFRP complex occurs mainly in sensory system. This suggested that complex could be targeted for the develop new analgesic strategy.
|
Report
(4 results)
Research Products
(9 results)
