An investigation on the role of HIF-1 in the regulation of microglia activity
| Project/Area Number |
24592336
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Kansai Medical University (2013-2014)
Kyoto University (2012) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIROTA Kiichi 関西医科大学, 医学部, 准教授 (00283606)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | マイクログリア / 低酸素誘導性因子1 / 神経因性疼痛 / 酸素代謝 / 自然免疫 / 慢性疼痛 / 脊髄 / 微小環境 / HIF-1 |
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| Outline of Final Research Achievements |
Microglia is a component equivalent to macrophage serving a critical innate immunity in the central nervous system (CNS). We demonstrate that in response to activation of the microglia cell line BV-2 cells, expression of both HIF-1a and HIF-1b; increases leading to enhanced expression of HIF-1 target genes. Moreover, we also demonstrated that spinal nerve ligation (SNL) in rats dysregulated the spinal oxygen homeostasis and that spinal hypoxia induced HIF-1 activation resulting in increase of mRNA expression of ATP receptors such P2X4 and P2Y7. Our results strongly suggest that increased activation of HIF-1 occurs in the CNS in response to spinal root ligation and that HIF-1 may play a critical role in the establishment of allodynia induced by SNL in which microglia activation is essential.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] UCHL1 provides diagnostic and antimetastatic strategies due to its deubiquitinating effect on HIF-1alpha2015
Author(s)
Goto Y, Zeng L, YeomCJ, Zhu Y, Morinibu A, Shinoyam K, Kobayashi M, Hirota K, Itasaka S, Yoshimura M, Tanimoto K, Sowa T, Menju T, Sonobe M, Kakeya H, Toi M, Date H, Hammond EM, Hiraoka M, Harada H.
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Journal Title
Nat Commun
Volume: 6
Issue: 1
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Involvement of decreased hypoxia-inducible factor 1 activity and resultant G(1)-S cell cycle transition in radioresistance of perinecrotic tumor cells2013
Author(s)
Y. Zhu, T. Zhao, S. Itasaka, L. Zeng, C.J. Yeom, K. Hirota, K. Suzuki, A. Morinibu, K. Shinomiya, G. Ou, M. Yoshimura, M. Hiraoka, H. Harada
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Journal Title
Oncogene
Volume: 32
Issue: 16
Pages: 2058-2068
DOI
Related Report
Peer Reviewed
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