| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Glucagon-like peptide-1 (GLP-1) is known to increase insulin secretion and play a crucial role in maintaining glucose homeostasis. In this study, we performed both in vivo and in vitro experiments to evaluate the interaction between GLP-1 and general anesthetics in rats. Our findings can be summarized as follows: 1) Inhalation of isoflurane led to a decrease in the basal levels of GLP-1. 2) Isoflurane attenuated the glucose-evoked increase in the levels of GLP-1 and insulin and increased the plasma glucose levels. 3) During oral glucose tolerance test, dexmedetomidine inhibited glucose-induced insulin secretion in dose-dependent manner. 3) In perifusion experiments, isoflurane inhibited glucose-induced insulin release, whereas exogenous GLP-1 enhanced it. 4) Whole-cell patches showed that GLP-1 could restore the glucose-stimulated depolarization suppressed by isoflurane. 5) Propofol has no effect on the GLP-1 release from intestinal L cells.
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