| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Outline of Final Research Achievements |
The purpose of the present research was to investigate the mechanisms of neuropathic pain and preventive effects of drugs by using in vivo patch-clamp technique in rat spinal dorsal horn and behavioral experiments. However, contrary to expectations, the synaptic property of substantia gelatinosa neurons in neuropathic pain model rat have not been clarified by the electrophysiological technique. In behavioral study, continuous intraperitoneal infusion of tramadol, dexmedetomidine, pravastatin and ketamine before the nerve injury had no preventive effect on neuropathic pain. Some preventive effects were observed by ropivacaine administered locally on ligated nerve. Further study will be needed to confirm this preventive effects. Our in vivo patch-clamp experiments revealed that systemic tramadol modulated the synaptic activities of substantia gelatinosa neurons in the spinal cord and indicated that metabolite M1 may play a key role in the antinociceptive mechanisms of tramadol.
|
