| Project/Area Number |
24592360
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UCHINO Hiroyuki 東京医科大学, 医学部, 教授 (60266476)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | ミトコンドリア / 肝不全 / 敗血症 / 国際情報交換 / スウェーデン |
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| Outline of Final Research Achievements |
To study the mechanism of SLF using the change of mitochondrial (Mito) respiratory function in SLF model. Male C57bl/6J mice were divided into two groups, one was CLP group induce sepsis and the other was Sham group. Isolated liver mito were exposed to continuous infusions of Ca2+. Monitoring of extra-mito Ca2+ and mito respiration provided a quantitative assay for mPT sensitivity by determining CRC and mito swelling%. And we investigated the role of different uncoupler and respiratory complex to evaluate maximal respiratory activity of mito and ETS. No significant changes in CRC, Swelling%, a value, CS and RCR of CLP were detected compared to Sham. The CLP tended to be slightly high in Swelling% rate and a value rate in CLP /Sham. FCCP and Rote value of CLP were lower compared to Sham significantly in SUIT protocol. There was little change in FCCP/Rot between both CLP and Sham. These results suggested the possibility of the obstacle of the ETS activity around complex I in CLP.
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